摘要
用新西兰白兔制作肠缺血再灌注模型,18只兔随机分为维拉帕米保护组(9只)和生理盐水对照组(9只)。观察缺血前、缺血60min 和再灌注30min 血清 LPO 及肠粘膜线粒体内钙浓度变化和肠粘膜损伤程度。结果显示:维拉帕米组血清 LPO 和线粒体内钙含量再灌注后低于对照组(P<0.01),肠粘膜组织学损伤程度比对照组轻(P<0.05)。表明线粒体内钙反常加重再灌注时氧自由基介导的组织损伤,维拉帕米对肠粘膜缺血再灌注损伤具有保护作用。
The purposes of this study are to confirm the effect of calcium and verapamil(VER)on intestinal ischemia—reperfusion injury.The model of intestinal ischemia—reperfusion was established in 18 New Zealand rabbits.The rabbits were randomly assigned into two groups,animals in the control group(n =9)received normal saline as placebo,in VER group(n=9)animals were treated with VER(2mg/. kg).Observation on the levels of serum LPO,the concentrations of calcium in mucosal mitochodria as well as the degree of mucosal injury before ischemia,60 minites after ischemia and 30 munites after reperfusin was conducted.We found that the concertrations of serum LPO and mitochonfrial calcium in VER group was lower than that in the control group after reperfusion(P<0.01).The intestinal mu- cosal injury was less severe in VER group compared with the control group(P<0.05).In conclusion, our findings indicate that calcium potentiated oxygen free radicals cause injury to intestinal mucosa dur- ing reperfusion.VER may protect intestinal mucosa from ischemia-reperfusion injury.
出处
《苏州医学院学报》
1996年第3期405-407,597,共3页
Acta Academiae Medicinae Suzhou
关键词
小肠缺血
再灌注损伤
钙
维拉帕米
Intestinal ischemia-reperfusion
calcium Verapamil
