中药提取物水苏糖对实验性肝硬化大鼠血浆内毒素及肠道菌群的影响

Study of the stachyose,an extract of Chinese herbal medicine, on pasm lipoplusaccharide endotoxin(LPS) and intestinal microflora in experimental liver cirrhosis rats

目的探讨中药提取物水苏糖对肝硬化大鼠血浆内毒素及肠道菌群的影响。方法36只SD大鼠随机分为正常对照组(n=6),模型组,预防组,晚期治疗组。采用CCL4复合因素法建立肝硬化模型;实验第9周末处死所有大鼠,观察药物对肝硬化大鼠内毒素,肠道菌群,AST、ALT肝功能及肝组织病理学等的影响。结果中药提取物水苏糖对内毒素及其它指标都有明显的改善,预防组,晚期治疗组病理都有改善,预防组优于治疗组。结论中药提取物水苏糖能有效调整肝硬化肠道菌群失调,并降低血浆内毒素。对肝脏有保护作用。

Objectives To study the effects of the stachyose on plasm lipoplysaccharide endotoxin （LPS） and intestinal microfiora in experimental liver cirrhosis. Methods 36 SD rats were divided into 4 groups randomly： normal group, preventive group,late treatment group and model group. Cirrhotic rats were induced by injecting Carbon tetrachloride（CC14）. At 9 weeks, the changes of LPS,intestinal microflora,AST,ALT hepatic function were measured and the pathology of liver were boserved by HE stain after the rats were sacrificed in the last injection of Carbon tetrachloride. Results LPS and the other indexes were improved by stachyoes. The pathological process of liver in preventive group and late treatment group were improved. The preventive group excelled the late treatment group. Conclusions Stachyose effectively adjust the imbalances of intestinal flora and reduce the level of blood endotoxin and protect hepatic cells.