矽肺结核病耐喹诺酮(gyrA)基因情况分析

Analysis of mutation of quinolones gyrA genes in silicotuberculosis

目的研究矽肺结核耐喹诺酮药敏试验与gyrA基因突变的关系。方法通过药敏试验和聚合酶链反应—单链构象多态性(PCR-SSCP)技术鉴定31株分枝杆菌临床分离株的菌种,分析gyrA基因突变的情况。结果30株临床分离株的16SrDNASSCP电泳图谱均与结核分枝杆菌标准株相同。16株耐喹诺酮类药临床分离株中,11株异常,占68.75%。结论gyrA基因突变与结核分枝杆菌对喹诺酮类药物耐药有关,且gyrA基因突变在药敏实验高、低浓度区均可发生。

Objective To study relevance of the mutations of quinolones gyrA genes in silicotuberculosis. Methods The mycobacterial species were identified in 31 clinical isolates. Meanwhile, the gyrA genes in the isolates were analyzed with PCR-SSCP. Results Thirty clinical isolates had the same 16S rDNA SSCP profiles as M. tuberculosis. Of the 16 quinolone resistant isolates, 11 （68.75%） displayed abnormal gyrA SSCP profiles. Conclusions Quinolones resistances in some M. tuberculosis isolates are due to mutations on gyrA genes, moreover, the mutation of gyrA genes can occur in isolate which has higher and lower resistant concentration.