摘要
介绍一种鱼藤酮的高效液相色谱定量分析方法。采用hypersil C18色谱柱,在以v(乙腈)∶v(水)=60∶40为流动相,流速为1.0 mL/m in,检测波长为294 nm的条件下,用外标法对鱼藤酮原药中的有效成分进行定量分析。方法的标准差为0.28,变异系数为0.39%,线性相关系数为0.9999,平均回收率为99.54%。
A HPLC method for the determination of rotenone was introduced. The test sample were separated on a hypersil C18 column during with ecetonitrile/water （60/40, V/V） at 1.0 ml/min flow ratio and detected at 294 nm. Resuits showed that standard deviation, variation coefficient and average recovery was 0.28, 0.39% and 99.54% respectively.
出处
《云南化工》
CAS
2006年第2期59-60,共2页
Yunnan Chemical Technology
