亚硒酸钠对全脑缺血再灌注脊髓损伤的保护作用

Preventive Action of Sodium Selenite on Cerebral Ischemia-reperfusion Damage to Spinal Cord

目的探讨亚硒酸钠对全脑缺血再灌注所致脊髓损伤的保护作用及其机制。方法家兔18只,随机分成假手术组,缺血再灌注组,亚硒酸钠预防组。采用改进的缺血再灌模型对家兔全脑缺血10 m in复流1 h,重复1次,术后12 h取材测定血清和脊髓组织匀浆中GSH-Px活力;电镜观察脊髓前角运动神经元形态的改变。结果亚硒酸钠预防组脊髓组织中GSH-Px活力明显增加,与缺血再灌注组比较有显著差异(P<0.01),血清中GSH-Px活力也明显增加,与缺血再灌注组比较有显著差异(P<0.01);电镜观察亚硒缺钠预防组能使脑缺血再灌注所脊髓前角运动神经元的损伤明显减轻。结论亚硒酸钠对缺血再灌注所致脊髓神经元损伤有明显的保护作用。

Objective To observe the preventive effect and mechanism of ample selenium on experimental cerebral ischemia reperfusion damage to spinal cord. Methods Eighteen rabbits were randomly divided into three groups： （ 1 ） sham operation group（ group A）, （2） ischemia reperfusion group（group B）, （3） ample selenium prevention. Our research used meliorative ischemia -reinfusion model. Brains of rabbits received ischemia l0 minutes, Reflowed 60 mins, done two times, twelve hours after the operation, the rabbits wre killed. The activity of GSH - Px in the spinal cord and serum was measured. The alter of structure in the motor neurons of the gray matter of spinal cord was observed with electron microsome. Results The activity of GSH -Px in the spinal cord and serum in the ample selenium group was high compared to that in the ischemia - reperfusion group（P 〈0.01 ）. Neurecyte structure observation showed that solidium selenite could relieve meurocyte damage caused by ischemia reperfusion in spinal cord, Conlusion Solidium selenite can obviously protect neurocytes from the damage caused by ischemia reperfusion.