过敏性紫癜肾炎患者血清对体外培养大鼠GMC增殖及活性氧产生的影响

Effect of the serum of patients with HSPN on the proliferation of mesangial cell and the yield of ROS

目的:探讨过敏性紫癜肾炎(HSPN)患者血清对体外培养大鼠肾小球系膜细胞(rGMC)增殖及活性氧(ROS)产生的影响。方法:分别采用HSPN和正常人血清体外培养rGMC,比较二者在细胞增殖及ROS产生上的差别。结果:HSPN组的rGMC增殖显著快于对照组(P<0.01);HSPN组超氧化物歧化酶值明显低于对照组,而产生活性氧单位则显著高于对照组(均为P<0.01)。结论:HSPN患者血清能显著刺激rGMC的增殖,且这一作用极可能与ROS产生过多相关。

Objective： To investigate the effect of the serum of patients with HSPN on the proliferation of rat glomerular mesangial Cell（rGMC） and the yield of ROS. Methods： The rGMC was cultured with the serum of patients with HSPN and normal controls, respectively. The prolifera-tion of mesangial cell and yield of ROS in the two groups were detected. Results： The proliferation of rGMC in HSPNgroup was significantly faster than that of the normal group（P〈0.01）. In HSPN group, the value of SOD was lower, while the unit of yield Ros was higher than those of the normal controls significantly （P〈0.01）. Conclusion： The serum of patients with HSPN has an evidently irritating effect on the rGMC proliferation. This effect may be attributed to the excessive yield of ROS.