摘要
以MS培养基为基本培养基,附加不同种类和浓度的生长调节物质,对不同器官,不同取材时期的重楼外植体进行愈伤组织的诱导培养研究.结果表明,BA和IAA有利于愈伤组织的诱导;生长期的幼芽组织是较适宜的培养器官,其愈伤组织的诱导频率达26.7%.该研究为进一步离体快繁完整植株打下了坚实的基础.
In the study, the explants of pairs of different organs and different growth duration were used to induce callus tissues on MS medium with different sorts and concentrations of growth regulating substances. The result indicated that 6-benzylaminopurine (6-BA) and IAA were beneficial to callus induction; Bud tissues in growth duration were proper explant and the percent of induction of callus was 26.7%. The study will provide a steady base for in vitro reproduction of whole plant in future.
出处
《四川师范大学学报(自然科学版)》
CAS
CSCD
北大核心
2006年第1期120-122,共3页
Journal of Sichuan Normal University(Natural Science)
基金
四川省教育厅青年科研基金资助项目
关键词
重楼属
愈伤组织
诱导
Pairs
Callus tissue
Induction