丝素蛋白膜仿生沉积磷灰石的实验研究

Study of Apatite Coating on Silk Fibroin Membrane by Biomimetic Mineralization.

目的:评价丝素蛋白的仿生矿化特性,为研制新型丝素蛋白/HAP复合生物材料提供实验依据。方法:采用氯化钙和磷酸氢二钠缓冲溶液交替矿化的方法,在丝素膜上仿生沉积羟基磷灰石。钙磷沉积物以SEM、EDX、XRD、FTIR进行检测。结果:随着矿化周期的增加,丝素膜上沉积的磷灰石数量逐渐增多、体积逐步增大、结晶度越来越高。矿化形成了针状类骨磷灰石晶体。晶体长约0.35μm,直径约30 nm。结论:交替矿化法是仿生构建丝素蛋白复合生物材料的有效方法。

Objective： The aim of this study was to evaluate the silk fibroin biomineralization behavior. Methods： Silk fibroin films were fabricated and biomimetic mineralization was carried out in the media of alternative CaCl2 and Na2HPO4 solutions （ACPS）. The formation of calcium phosphate deposit was identified by scanning electron microscopy （SEM）, energy - dispersive X - ray （ EDX）, X - ray diffraction （XRD） and Fourier transform infrared spectroscopy （FTIR）. Results： Calcium - deficient apatite was deposited on the surface of fibroin film. After soaking for three cycles, the needle - shaped crystals covered the whole area of the film. The bone - like apatite was well - crystallized with the crystal length of about 0. 35 μm and the diameter of some 30nm. Conclusion ： A quick formation of hydroxyapatite on the surface of the silk fibroin film indicates that ACPS may be an effective approach to obtaining fibroin/HAP composites.