期刊文献+

骨髓间充质干细胞向前列腺癌趋向转移的研究 被引量:13

Directed migration of human mesenchymal stem cells to prostate cancer microenviroment
原文传递
导出
摘要 目的探讨人骨髓间充质干细胞(hMSCs)在体内向前列腺癌微环境趋向转移的特性。方法应用人前列腺癌细胞株PC-3异种皮下种植建立前列腺癌严重联合免疫缺陷小鼠 (SCID)模型,通过密度梯度离心和贴壁法分离、培养hMSCs,将体外4,6-联脒-2-苯基吲哚(DAPI) 标记的4~6代hMSCs经尾静脉和肿瘤周围注射入荷瘤SCID鼠体内,分别在经尾静脉注射后17 d 和肿瘤周围注射后7、10、14 d处死小鼠,收集肿瘤和肝、肺、脾、肾等脏器作冰冻切片和石蜡切片, 在荧光显微镜下观察肿瘤及各脏器中hMSCs的分布情况。结果前列腺癌SCID鼠模型成瘤率 83.3%,经尾静脉注射DAPI标记的hMSCs后17 d和肿瘤周围注射DAPI标记的hMSCs后7、10、 14 d,荧光显微镜下显示,前列腺癌组织中可见DAPI标记的hMSCs的核呈蓝色荧光,而肝、肺、脾、肾等脏器中均未见hMSCs的存在。结论 hMSCs在体内具有向前列腺癌微环境趋向转移的特性。 Objective To investigate the migration behavior of human mesenchymal stem cells (hMSCs) in response to prostate cancer microenviroment in vivo. Methods The human prostate cancer cell line PC-3 was injected into the flank or axillary of severe combined immunodeficiency (SCID) mice subcutaneously to establish human prostate cancer xenograft models, hMSCs were harvested from donor's ribs of human cadaver renal transplantation and separated by density gradient centrifuge, hMSCs between passages 4 to 6 were labeled with 4', 6-diamidino-2-phenylindole (DAPI). DAPI-labded hMSCs were injected into the bearing cancer SCID mice via tail vein or 0.5 cm away from peritumor subcutaneously. Seventeen days after injection via tail vein, and 7, 10 and 14 days after injection by peritumor subcutaneously, the mice were killed and their tumors, livers, lungs, spleens and kidneys were harvested. Frozen sections and paraffin-embedded sections were used to observe the distribution of exogenous DAPI-labeled hMSCs in vivo by a fluorescence microscope. Results In SCID mice injected with PC-3 subcutaneously, the tumorigenic rate was 83.3 %. The tumors were identified by pathology. Seven days after injection of hMSCs via tail vein, and 7, 10 and 14 days after injection of hMSCs by peritumor subcutaneously, DAPI-labeled hMSCs with blue nuclei were distributed extensively in the tumors. But no blue nucleus were seen in the livers, lungs, spleens and kidneys. Conclusion Exogenous purified hMSCs can migrate to prostate cancer mi t in vivo.
出处 《中华实验外科杂志》 CAS CSCD 北大核心 2006年第5期582-584,i0004,共4页 Chinese Journal of Experimental Surgery
基金 国家自然科学基金资助项目(30560153)
关键词 骨髓间充质干细胞 前列腺癌 转移 Mesenchymal stem cells Prostate carcinoma Metastasis
  • 引文网络
  • 相关文献

参考文献14

  • 1张正望,殷莲华,张永康,赵凤娣.C57BL6近交系小鼠前列腺癌原位移植瘤模型的建立[J].中华实验外科杂志,2001,18(5):413-414. 被引量:5
  • 2Henry N, Van Lamsweerde AL, Vaes G. Collagen degradation by metastatic variants of Lewis lung carcinoma: cooperation between tumor cells and macrophages. Cancer Res, 1983, 43:5321-5327.
  • 3Asahara T, Mesuda H, Takaheshi T, et al. Bone marrow origin of endothelial progenitor cells responsible for postnatal vasculogenesis in physiological and pathological neovescularization. Circ Res, 1999, 85:221-228.
  • 4Ishii G, Sangai T, Oda T, et al. Bone-marrow-derived myofibroblasts contribute to the cancer-induced stromal reaction. Biochem Biophys Res Communk, 2003, 309: 232 -240.
  • 5Studeny M, Marini FC, Champlin RE, et al. Bone marrow-derived masenchymal stem cells as vehicles for interferon-beta delivery into tumors.Cancer Res, 2002, 62 : 3603-3608.
  • 6鲁玲玲,刘玉军,孙晓红,刘华松,孙正,杨慧.骨髓间充质干细胞在大鼠体内的迁移研究[J].中国生物工程杂志,2005,25(4):22-28. 被引量:5
  • 7Pittenger MF, Mackay AM, Beck SC, et al. Multilineage potential of adult human mesenchymal stem cells. Science, 1999, 284 : 143-147.
  • 8李志刚,梅举,张宝仁.骨髓基质细胞移植于缺血心肌的研究[J].中华实验外科杂志,2002,19(5):436-437. 被引量:6
  • 9Sordi V, Malosio ML, Marchesi F, et al. Bone marrow mesenchymal stem cells express a restricted set of functionally active chemokine receptors capable of promoting migration to pancreatic islets. Blood,2005, 106 : 419-427.
  • 10Sun YX, Wang J, Shelburne CE, et al. Expression of CXCR4 and CX-CL12 (SDF-1) in human prostate cancers (PCa) in vivo. J Cell Biochem, 2003, 89:462-473.

二级参考文献28

  • 1Wang X E,The Prostate,1999年,39卷,182页
  • 2An Z L,The Prostate,1998年,34卷,169页
  • 3Woodbury D, Schwarz E J, Prockop D J, et al. Adult rat and human bone marrow stromal cells differentiate into neurons. J Neurosci Res, 61:364 ~ 370.
  • 4Pereira R F, Halford K W, O' Hara M D, et al. Cultured adherent cells from marrow can serve as long-lasting precursor cells for bone, cartilage, and lung in irradiated mice. Proc Natl Acad Sci USA, 1995, 92(10): 4857~4861.
  • 5Prockop D J. Marrow stromal cells as stem cells for nonhematopoietic tissues. Science, 1997, 276(4): 71~74.
  • 6Sanchez-Ramos J, Song S, Cardozo-Pelaez F, et al. Adult bone marrow stromal cells differentiate into neural cells in vitro.Experimental Neurology, 2000, 164:247~256.
  • 7Prockop D J, Azizi S A, Colter D, et al. Potential use of stem cells from bone marrow to repair the extracellular matrix and central nervous system. Biochem Soc Trans, 2000, 28(4): 341 ~345.
  • 8Piersma A H, Brockbank K G, Ploemacher R E, et al.Characterization of fibroblastic stromal cells from murine bone marrow. Exp Hematol, 1985, 13:237~243.
  • 9Friedenstein A J, Chajlakjan R K, Latzinik N W, et al. Stromal cells responsible for transferring the microenvironment of the hemopoietic tissuses: cloning in vitro and transplantation in vivo.Transplantation, 1974, 17:331~340.
  • 10Tavassoli M, Takahashi K. Morphological studies on long-term culture of marrow cells: characterization of the adherent stromal cells and their interactions in maintaining the proliferation of hemopoietic stem cells. The American Journal of Anatomy, 1982,164:91 ~ 111.

共引文献13

同被引文献190

引证文献13

二级引证文献22

;
使用帮助 返回顶部