红树林放线菌0616167的生长特性及发酵条件优化

Growth Characteristics and Fermentation Condition Optimization of Mangrove Actinomycete Strain 0616167

菌株0616167是分离自红树林的具细胞毒活性的放线菌。研究表明，该菌最适生长的NaCl浓度是6％，能够耐受最高的NaCl浓度为20％；当盐度为0～3％时，细胞毒活性最高。通过正交试验法对发酵条件的优化，得出有利于细胞毒活性的最佳碳源、氮源、通氧量及发酵时间分别为：葡萄糖20g，硝酸钾15g，装液量为75mL/250mL三角瓶，发酵时间5d。通过优化，细胞毒活性提高了3倍。

Strain 0616167 was an actinomycete with cytotoxicity isolated from sea mud of mangrove. Optimum concentration of NaCl for this strain is 6%, and the highest tolerance of NaC1 is 20%. The highest cytotoxic activity was obtained at 0 - 3% of NaCl. The optimum condition for cytotoxity production of strain 0616167 is determined by orthogonal design. Three times higher activity was obtained at the carbon sourse, nitrogen source, oxygen supply and fermentation time was： 20g of glucose, 15g of KNO3 , 75mL media in 250mL flasks and 5 days of fermentation time, respectively.