谷氨酰胺诱导大鼠热休克蛋白70mRNA的表达

Glutamine induces the expression of HSP70 mRNA in the rat

Objective To observe the expression of HSP70 mRNA in the rat induced by glutamine.Methods ①Twenty four healthy Sprague-Dawley rats were randomly divided into four groups（n=6 in each group）: groupⅠ received saline i.v.;group Ⅱ received glutamine（Gln） 0.15 g·kg-1 i.v.;group Ш received Gln 0.45 g·kg-1 i.v.;group Ⅳ received Gln 0.75 g·kg-1 i.v..All rats were killed after 4 h administration of Gln.HSP70 mRNA from heart、liver、lung and aorta as well as GAPDH mRNA were measured by reverse transcription polymerase chain reaction（RT-PCR）.② Thirty healthy Sprague-Dawley rats were randomly divided into five groups（n=6 in each group）.They were killed at 1 h（group 1 h）、6 h（group 6 h）、12 h（group 12 h）、24 h（group 24 h） and 48 h（group 48 h） after administration of Gln(0.75 g·kg-1).HSP70 mRNA from heart、liver、lung and aorta as well as GAPDH mRNA were measured by reverse transcription polymerase chain reaction（RT-PCR）.Results ① The expression of HSP70 mRNA in group Ⅱ、Ш and Ⅳ were higher than groupⅠ（P<0.05）,and the expression in groupⅡ、Ш and Ⅳ increased gradually.②The expression of HSP70 mRNA in group 1 h、 6 h and 12 h was higher than that in 24 h and 48h（P<0.05）.Conclusion Glutamine induces the expression of HSP70 mRNA in rats,and it occurs with the manner of dose and time dependent.

Objective To observe the expression of HSP70 mRNA in the rat induced by glutamine. Methods ①Twenty four healthy Sprague-Dawley rats were randomly divided into four groups （n =6 in each group） ： groupI received saline i.v. ;group Ⅱ received glutamine （Gin） 0. 15 g · kg^-1 i. v. ; group Ⅲ received Gin 0.45 g · kg^-1 i. v. ; group IV received Gin 0.75 g · kg^-1 i.v.. All rats were killed after 4 h administration of Gin. HSP70 mRNA from heart,liver,lung and aorta as well as GAPDH mRNA were measured by reverse transcription polymerase chain reaction （RT-PCR）.②Thirty healthy Sprague-Dawley rats were randomly divided into five groups （n = 6 in each group）. They were killed at 1 h （group 1 h） ,6 h （group 6 h） ,12 h （group 12 h） ,24 h （group 24 h） and 48 h （group 48 h） after administration of Gin （0.75 g · kg^-1 ）. HSP70 mRNA from heart,liver,ltmg and aorta as well as GAPDH mRNA were measured by reverse transcription polymerase chain reaction （RT-PCR）. Results ③ The expression of HSP70 mRNA in group Ⅱ,Ⅲ and Ⅳ were higher than groupI （ P 〈 0. 05 ）, and the expression in groupⅡ,Ⅲ and Ⅳ increased gradually. ②The expression of HSP70 mRNA in group 1 h, 6 h and 12 h was higher than that in 24 h and 48h （P 〈0.05）o Condusion Glutamine induces the expression of HSP70 mRNA in rats, and it occurs with the manner of dose and time dependent.