摘要
探索超滤法分离浮萍中免疫活性成分浮萍多糖的工艺.通过煎煮、超滤、脱蛋白、乙醇沉淀等步骤对浮萍多糖进行提取分离,硫酸-苯酚法和硫酸-蒽酮法测定提取液和样品中的总糖含量,考马斯亮蓝G250比色法测定其蛋白质含量.得到浅褐色粉末,纯度53.5%,蛋白质含量1.2%,收率6.43%.该方法可以简单、快速、有效地对浮萍多糖进行分离.
The aim of the paper is to investigate the technics of isolating the immune-active polysaccharides from Herba Spirodelae seu Lemnae (PHS) by ultrafihration. PHS were extracted and isolated through decoction, ultrafiltration, removal of protein and ethanol precipitation et al. The total contents of saccharides in the extraction and sample were determined by the methods of sulphuric acid phenol and sulphuric acid anthrone. The protein contents were determined by colorimetric method of coomassie brilliant blue G 250. Some hazel powder was gained. The purity, the contents of protein, and the recovery are 53.5%, 1.2%, and 6.43%, respectively. Ultrafiltraion is a kind of method, which can isolate PHS simply, quickly and effectively.
出处
《重庆大学学报(自然科学版)》
EI
CAS
CSCD
北大核心
2006年第4期69-72,共4页
Journal of Chongqing University
关键词
浮萍
多糖
分离
超滤
Herba Spirodelae seu Lemnae
polysaccharides
isolate
ultrafiltration