高效液相色谱法测定雷公藤饮片中雷公藤内酯醇的含量

Content Determination of Triptolide in Tripterygium Wilfordii Cut Crude Drugs by HPLC

目的:建立以高效液相色谱(HPLC)法测定雷公藤饮片水提物中雷公藤内酯醇含量的方法。方法:样品分别用氯仿和乙酸乙酯超声萃取,经中性氧化铝柱洗脱分离后,在流动相为乙腈-水(23∶77)、流速为1.0ml/min、检测波长为218nm条件下测定含量并进行比较。结果:雷公藤内酯醇检测浓度线性范围为2.74～96μg/ml(r=0.9999),平均回收率分别为94.78%(RSD=1.57%)、98.2%(RSD=1.7%)。结论:2种含量测定方法均可用于雷公藤内酯醇的质量控制,尤以乙酸乙酯处理样品的方法分离、测定效果较好。

OBJECTIVE：To develop a RP HPLC method for the determination of triptolide in tripterygium wilfordii cut crude drugs.METHODS： Samples were extracted with chloroform and acetic ether by ultrasound and underwent neutral alumina column elution and isolation, the content determination was performed with acetonitrile water（23 ： 77） as mobile phase at a flow rate of 1.0ml/min and detection wavelength at 218nm.RESULTS： The linear detection concentration range of triptolide alcohol was 2.74-96μg/ml（ r = 0.9 999）, and the average recovery were 94.78% （RSD= 1.57% ） and 98.2% （RSD = 1.7% ）, respectively.CONCLUSION：Both methods are applicable for the quality control of triptolide alcohol, yet the separation and determination outcome were better for the sample treated with acetic ether.