摘要
文中采用网袋容器和自动间歇喷雾扦插繁殖技术系统地研究了祁连圆柏插穗生根的外在因素和生理生化基础,对祁连圆柏插穗母株年龄、长度、类型、激素处理等对插穗生根的影响进行了试验.采用高效液相色谱法测定不同插穗类型内源激素(IAA、ABA、GA3、Z、ZR、iPA)、酚类化合物、糖类的含量.结果表明:硬枝插穗随着母株年龄增长,生根率下降;激素处理能显著提高硬枝插穗的生根率,其中以IBA 500 g.kg-1处理生根效果最好,生根率达到75%以上;长度小于10 cm的插穗生根率高;不同枝条类型之间的生根能力没有显著差异;3种插穗类型的IAA、Z、GA3、酚类化合物和糖类及IAA/ABA、IAA/ZT的比值差异较大,单一的某种激素对祁连圆柏插穗生根没有明显影响.
The purpose of the study was to investigate the techniques of cutting propagation and physiological and biochemical basis for Sabina przewalskii. By cutting method in auto-interval mist system, together with some treatments, the rooting ability of different ortet ages, different cutting length, different branches types, different plant hormones, some rooting index such as rooting ratio, rooting quantity and root length were investigated. The endogenous hormones content, including IAA, ABA, GA3 and Z, phenol compounds and sugar compound were measured using HPLC. The result obtained by the experiment indicated that the rooting ratio of young individuals was higher than mature individuals'; The rooting ratio of hardwood was increased by plant hormone treatment, especially treated by IBA 500kg^-1, the rooting ratio has been over 75% ; The best cutting length is less than 10 cm; There is no difference of rooting ratios between branches types. The content of IAA, ABA, GAs and Z, phenol compounds and sugar compound as well as the ratio of IAA to ABA, IAA to Z from different cutting types are correlated with the rooting ratio. Single hormone has no obvious effect on the rooting of hardwood cutting of S. przewalskii.
出处
《福建林学院学报》
CSCD
北大核心
2006年第2期135-139,共5页
Journal of Fujian College of Forestry
基金
退耕还林科技支撑专题"祁连山退耕区植物新品种的引进和乡土树种的扩繁技术研究"
国家林业局重点项目"西部干旱区设施育苗技术与试验示范"(2003-027-L27)
关键词
祁连圆柏
硬枝扦插
生根率
生理生化基础
Sabina przewalskii
hardwood cutting
rooting rate
physiological and biochemical basis