摘要
通过正交实验探讨了微波辅助法提取柿叶黄酮的最佳条件.利用3种大孔吸附树脂对柿叶黄酮的静态吸附实验筛选了最优的吸附分离树脂,并确定了乙醇洗脱最优体积分数.结果表明最佳提取条件为料液比1∶30(m∶v),微波强度250W,提取时间21min;DA-201型大孔吸附树脂对柿叶黄酮吸附效果最好且70%乙醇解吸率最高.
The best extraction conditions for the extraction of total flavonoids by microwave-assisted extraction technique from Diospyros kaki leaf was investigated. The influence of microwave power, extraction time and the ratio of solidliquor on the extraction efficiency was discussed. Total flavonoids was quantified by UV method. Three types of macroreticular resin with'different polarity were compared in the adsorption ability to flavonoids compounds of Diospyros kaki leaf. The optimum absorbent for total flavonoids in Diospyros kaki leaf was decided to be DA-201. The best ratio of solid-liquor was 1 : 30. The highest extraction yield of flavonoids in Diospyros kaki leaf was obtained by extracting for 21min using 250 W microwave power.
出处
《吉首大学学报(自然科学版)》
CAS
2006年第1期110-113,共4页
Journal of Jishou University(Natural Sciences Edition)
关键词
柿叶
黄酮
微波辅助法
大孔吸附树脂
正交实验
Diospyros kaki leaf
flavonoids
microwave-assisted extraction
macroreticular resin
orthogonal factoral design