支原体颗粒抗小儿肺炎支原体感染的实验研究

The experimental study of mycoplasma granule for children pneumonia infected by mycoplasma

目的:从实验方面对支原体颗粒治疗小儿肺炎支原体感染的疗效和作用机制进行系统的观察和研究。方法:选用Wistar大鼠,制作肺炎支原体感染模型;选昆明系小鼠制作相应模型,用支原体颗粒与利君沙对照。药效学研究:1.观察支原体颗粒对感染后大鼠肺炎支原体的清除作用。2.观察支原体颗粒对皮下注射内毒素粗制液后小鼠肛温变化的影响,研究支原体颗粒的解热作用。3.采用二甲苯致小鼠耳廓肿胀法研究支原体颗粒的抗炎作用。4.采用氨气刺激法研究支原体颗粒对小鼠的止咳作用。5.腹腔注射酚红溶液,研究支原体颗粒的化痰作用。药理学研究:1.观察感染后大鼠T细胞亚群、血液流变学和肺部病理的变化。2.观察感染后大鼠血清一氧化氮(NO)和丙二醛(MDA)含量的变化。结果:支原体颗粒有显著的抗肺炎支原体、解热、抗炎、止咳、化痰作用,并有显著的抗氧化、活血化瘀、改善肺部病理表现、调节免疫功能的作用。结论:支原体颗粒对小儿肺炎支原体感染具有良好的治疗作用。

Objective： Made a systematic observation and study in the curative effect and mechanism of the mycoplasma granule （MG） for children pneumonia infected by mycoplasma. Methods： Established pneu- monia model infected by mycoplasma by selecting rats and mice. Then observed the elimination of MG for pneumonia mycoplasma mice. At the same time, in rats. Studied the antipyretic effect of MG by hypodermic injecting endotoxin in observed the anti-inflammatory effect and the function of relieving cough and reducing sputum of MG in mice. In pharmacology respect, studied T cell subpopulation, blood rheology, pathologitc change of the lung, NO and MDA in blood in rats. Results： MG had remarkable effects in resisting pneumonia mycoplasma, antipyret, anti-inflammatory, relieving cough and reducing sputum. Meanwhile, MG had functions in antioxidation, promoting blood circulation to remove blood stasis, improving pathologitc change of the lung, regulating immunologic function. Conclusion： MG has obvious curative effects for children pneumonia infected by mycoplasma