摘要
在pH 2.5的酸性介质中, 蛋白质与偶氮胂Ⅲ-镧(Ⅲ)络合物能够相互作用, 形成超分子复合物而使溶液褪色. 最佳条件下, 在650 nm处吸光度的降低值, 与加入的牛血清白蛋白(BSA)或人血清白蛋白(HSA)、溶菌酶(Lyso)质量浓度在一定范围内呈线性关系. BSA在0.5~35 mg/L、 HAS在1~40 mg/L, 溶菌酶(Lyso) 1.7~45 mg/L, 相对标准偏差为0.82% (n=7). 方法用于人血清总蛋白量的测定.
ArsenazoⅢ-Lanthanum( Ⅲ ) can react with proteins to produce supermolecular complexes Arsenazo Ⅲ-Lanthanum( Ⅲ )-BSA in the buffer solution of citrate sodium-HCl (pH 2.5). The binding interaction is completed within 2 min at room temperature, and causes absorbance decrease at 650nm of the Arsenazo Ⅲ-Lanthanum( Ⅲ) complex. The decrease of absorbance is proportional to concentration of proteins. The linear range is from 0.5 to 35 mg/L for BSA, from lto 40 mg/L for HAS, from 1.7 to 45 mg/L for Lyso. The method has been applied to the determination of protein in human serum albumin samples with satisfactory results.
出处
《分析试验室》
CAS
CSCD
北大核心
2006年第5期91-93,共3页
Chinese Journal of Analysis Laboratory
基金
中南民族大学自然科学基金(YZZ03003)
湖北省自然科学基金(2005ABA001)