金银花在体内抗氧化作用的实验研究

Anti-oxidative effect of honeysukle in vivo: An experimental study

目的探讨金银花在体内的抗氧化作用。方法将20只Wistar大鼠随机分为两组。其中,实验 1组(大剂量组10只)予金银花水煎液(10g／kg)灌胃;实验2组(小剂量组10只)予金银花水煎液(5g／kg) 灌胃;另设对照组(10只)予蒸馏水灌胃。分别在灌胃前、灌胃后1、2h由眼眶取血,分离血浆,检测不同时间血浆内T-AOC、GSH-Px、GSH、MDA、NOS、NO、SOD的变化。结果实验1组和实验2组与对照组相比,灌胃1、2h后T-AOC、GSH-Px、GSH、SOD都有明显增加,而NOS和NO无明显变化,MDA明显下降; 1h时增加或减少幅度大于2h。结论金银花可提高体内抗氧化作用,且有剂量依赖性。

Objective To explore the anti-oxidative effect of honeysukle in vivo.Methods The 30 Wistar rats were random divided into 3 groups： high dose group （10g/kg,the decoction,given into stomach）（H）, low dose group（Sg/kg）（S）, and the control group（distilled water） （C）. The alteration of TAOC,SOD,GSH-Px,GSH,NOS,NO and MDA in the plasma was detected before and later 1h and 2h after garage administration of the honeysukle decoction.Results The rehtive items（see following mentioned） of H and S groups were compared with C group. The plasma levels of T-AOC, SOD, GSH-Px, GSH were increased, MDA was decreased,and NOS and NO had no significant change later lh and 2h after administration of the decoction.Conclusion Honeysukle could enhance anti-oxidative effects in vivo,and is of dependent on dosage.