甜瓜杂交种SSR指纹图谱的构建

Establishment of fingerprinting for melon hybrids by SSR markers

以2个甜瓜杂交品种(系)的种子东方蜜1号和01-31为试材,从63对SSR引物中筛选出10对扩增产物具有稳定多态性的引物,这些引物分布在甜瓜整个基因组,每对引物可以检测到2～4个数目不等的多态性片段(allele),共27个多态性片段,平均为2.7个,片段大小介于85￣270bp之间,并从入选的10对SSR引物中选出7对构建了这2个甜瓜杂交种的指纹图谱。在比较分析各试材图谱的基础上,探讨了品种指纹图谱的统计学方法,并对构建的2个甜瓜杂交种的指纹图谱进行了可信度分析,建立了适于种子检验中使用的标准SSR标记指纹图谱。实验表明,利用SSR技术进行甜瓜杂交种的纯度鉴定是可行的、有效的。

Two melon hybrids （Dongfangmi No.1 and 01-31） were analyzed by SSR marker to establish the standard DNA fingerprinting for seed test. Among 63 pairs of primers, 10 pairs with steady polymorphism were selected and distributed in melon genome. 2 alleles to 4 alleles were detected by each pair primers. There were 27 alleles detected in total. The average number of alleles detected was 2.7 per pair primer. The band size ranged from 85 to 270 bp. Finally, 7 out of 10 pairs of SSR primers were selected, which established fingerprinting for two hybrids. According to the data above mentioned, the fingerprinting of the melon hybrids was established and counted firstly. Then the paper introduced the method to calculate probability for the fingerprinting of each hybrid, and finally the standard, exclusive fingerprinting of melon hybrids was determined to distinguish the truth and falseness of samples in seed test. The experiment showed that SSR technique was practical and effective to identify melon hybrids.