龙胆泻肝汤中关木通配伍的肾毒性研究

The Nephrotoxicity of Long Dan Xie Gan Decoction and the Relevance with Aristolochia manshriensis Kom in the Decoction

目的:观察龙胆泻肝汤中关木通配伍与大鼠肾损伤的关系。方法:大鼠随机分为含关木通的龙胆泻肝汤(17.5g/kg)组、龙胆泻肝汤去关木通(11.5g/kg)组、单味关木通(6g/kg)组和正常对照组四组,前三组分别含关木通6g/kg给以相应方药水煎液灌胃,连续12周,对照组给予等量蒸馏水。于给药第4、8、12周分别测定各组大鼠肾功能及尿生化指标。结果:单味关木通(6g.kg-1)和含相同剂量关木通的龙胆泻肝汤连续给大鼠灌胃12周,均见有肾损伤表现,其中龙胆泻肝汤组损伤较单味关木通组轻;去关木通的龙胆泻肝汤连续给大鼠灌胃12周,未见肾功能明显损伤。结论:实验剂量的关木通可引起肾损伤,龙胆泻肝汤的肾毒性与方中关木通配伍相关,复方配伍对关木通的肾损伤可能有一定的减毒的作用。

Objective：To observe the renal injury in rats induced by Long Dan Xie Gan decoction（LDXGD） and the compatibilition with Aristolochia manshriensis Kom in LDXGD. Method：Separate SD rats to four groups at random, and feed respectively LDXGD containing Aristolochia manshriensis Kom （ 17.5g·kg^-1, containing Aristolochia manshriensis Kom 6g·kg^-1）, LDXGD removed Aristolochia manshriensis Kom （11.5g·kg^-1）, Aristolochia manshriensis Kom （6g·kg-^1） and distilled water for 12 weeks.determine renal functional parameters on 4w,8w, 12w. Result： Both Aristolochia manshriensis Kom （6g·kg^-1） and the LDXGD containing same dose Aristolochia manshriensis Kom induced significantly renal injury in rats during 12 weeks, and the former caused more seriously damage; the LDXGD removed Aristolochia manshriensis Kom didn＇t induce damage obviously in rats during 12 weeks. Conclusion： Aristolochia manshriensis Kom in experimental dosage can induce renal injury, and the real toxicity of LDXGD is correlated with the dose of Aristolochia manshriensis Kom in itself;the compatibilition in the complex prescription（LDXGD） relieve possibly the renal toxicity of Aristolochia manshriensis Kom.