测定胆固醇含量鉴别地沟油的研究

Distinguishing hogwash oil in edible vegetable oil by determination of cholesterol content

提出了用气相色谱法测定胆固醇含量鉴别地沟油的方法。地沟油是从餐饮业废弃物中提取的,成分复杂,常混有动物脂肪。动物脂肪中普遍含有大量的胆固醇,而在植物油中一般不含或含有极少量的胆固醇。植物油中主要含有谷甾醇、豆甾醇、菜油甾醇等多种植物甾醇。利用极性毛细管色谱柱可将油脂中的胆固醇和植物甾醇很好地分离,从而可鉴别油样中是否混有地沟油。使用这种方法,当食用植物油中掺有10%(质量分数)以上的地沟油时,即可检出。

Hogwash oil is collected and refined from eatery offal, and it always contains animal fat. The tissue and the fat of animal generally contain abundant cholesterol, however the vegetable oil commonly contain no or thimbleful cholesterol which can not be detected. A gas chromatographic method for determination of cholesterol was applied to distinguish hogwash oil from the edible vegetable oil. A polar capillary column was used to separate the cholesterol from phytosterol. The results showed that when hogwash oil into edible oil upwards of 10%, the charac- teristic peak of cholesterol could be observed obviously, ff the content of cholesterol was more than 0.05 mg/g, the vegetable oil was considered to have mixed with hogwash oil.