冬虫夏草菌丝体水提液对SP2/0细胞周期的作用

Effects of Water Extract from Cordyceps sinensise Mycelium on SP2/0 Cell Cycles

用四唑篮比色法试验,琼脂糖凝胶电泳方法及流式细胞仪研究了冬虫夏草菌丝体水提液对小鼠骨髓瘤细胞生长、凋亡及细胞周期的影响。结果发现浓度为100mg/ml水提液抑制瘤细胞的增殖,抑制率为70%,使细胞周期阻滞,在DNA电泳出现明显凋亡带,这提示水提液对瘤细胞抑制作用可能是由于细胞周期阻滞诱导细胞凋亡的结果。

After the SP2/0 cells had been treated with water extract from Cordyceps sinensise mycelium in different concentration, the effects on the growth, apoptosis, and cell cycles of the tumour cells were studied with MTT assay, agarose gel electrophoresis and flow cytometry, respectively. Results showed that the water extract （50mg/ml） has a relatively strong inhibitor effect on the proliferation of SP2/0 cells with restrain rate 70%. A typical DNA ladder was detected by agarose gel electrophoresis of DNA extracted from SP2/0 cells. Besides, the tumour cells were found to be blocked in the G0/G 1 phase. It suggested that the inhibition of the tumour cells by water extract may be related to inducing the apoptosis and blocking the tumour cells in the G0/G1 phase.