HPLC同时测定心脑治胶囊中三七皂苷R_1、人参皂苷Rg_1、人参皂苷Rb_1含量

Determination of notoginsenoside R_1,ginsenoside Rg_1 and Rb_1 in Xinnaozhi Capsule by HPLC

目的：建立测定心脑治胶囊（三七，水蛭，土鳖虫）中3种皂苷类成分三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1含量测定方法。方法：采用HPLC法，使用C18柱，流动相为乙腈-水梯度洗脱，0—3min，20％-25％乙腈，3—15min，25％-45％乙腈；检测波长为203nm；室温。结果：此方法能使样品中的3种皂苷类成分得到良好分离，且线性关系良好，平均加样回收率：三七皂苷R1为102．93％，RSD为1．26％；人参皂苷Rg1为105．68％，RSD为1．52％；人参皂苷Rb1为104．34％，RSD为0．70％。结论：本法简便、快速。结果令人满意，可用于作为心脑治胶囊质量控制方法之一。

AIM： To develop a method of determining notoginsenoside R1, ginsenoside Rg1 and Rb1 contents in Xinnaozhi Capsule （ Radix et Rhizoma Notogineseng, Hirudo, Eupolyphaga seu steleophage）. METHODS ： HPLC was used to determine notoginsenoside R1 , ginsenoside Rg1 and Rb1 in Xinnaozhi Capsule. The separation was performed by Zorbax C18 eolunm with aeetonitrile and water using a gradient program at UV 203nm at room temperature. The elution program was 0 - 3 min, 20% - 25% aeetonitrile, 3 - 15 min, 25% - 45% aeetonitrile. RESULTS： 3 saponins were separated well. Average recoveries were 102.93% for notoginsenoside R1 （RSD = 1.26% ） ; 105.68% for ginsenoside Rg1 （RSD = 1.52% ） ; 104.34% for ginsenoside Rb1 （RSD = 0.70% ）, respectively. CONCLUSION ： The method is simple and rapid and with satisfactory results. It is suitable for quality control of Xinnaozhi Capsules.