山核桃种仁蛋白质及氨基酸成分含量的变异分析

Analysis on the Protein and Amino Acids Contents in Hickory(Carya cathayensis Sarg.)Kernel and Their Variations

以3个地区50个山核桃初选优树为试样,研究了地域分布和单株在种仁粗蛋白、氨基酸组成和氨基酸含量诸性状上的差异。结果表明:(1)临安、淳安、安吉三地区山核桃种仁粗蛋白含量平均值为113.0 mg.g-1,变异系数为9.73%;方差分析表明地域分布对粗蛋白含量的影响达到显著水平,具中等广义遗传力(H2=0.342 9)。(2)山核桃种仁富含17种氨基酸,其中含人体8种必需氨基酸中的7种,必需氨基酸占氨基酸总量的33.07%,并且比例是稳定的,变异系数仅1.63%;而氨基酸总量以及7种必需氨基酸的含量变异系数较大,均超过16%,尤以甲硫氨酸为最,达到64.92%。

The study was carried out to test the variations of different regionas and individual trees in crude protein content and amino acid compositioh of hickory（ Carya cathayensis） kernel based on 61 plus trees from 4 areas（ Lin＇an ,Chun＇an, Anji and Ningguo）. The results showed that the crude protein content was 113.0 mg·g^-1 with the variation coefficient of 9.73% ;The variance analysis indicated that the variations between regions in crude protein contents were significant, and the broad-sense heritability （H2 ） was 0. 3429 . The hickory kernel contained 17 kinds of amino acids, including seven kinds of essential amino acids, which took 33.07% of the total amino acid contents. The variation coefficients of total amino acids and seven essential amino acids contents were large, and that of methionine was the largest, reaching to 64.9% . These demonstrated that the selection of superior hickory strains was genetically possible.