不同浓度的FBS和hLIF对小鼠原核期受精卵体外发育率的影响

Effect of the FBS and hLIF of Different Concentrations on the Viability of Mouse Pronuclear Zygote in Vitro

通过在mM16培养液中添加不同浓度的FBS及hLIF,探讨小鼠原核期受精卵的体外培养条件,旨在建立稳定的小鼠原核期受精卵的体外培养系统。实验结果表明:在mM16中添加10%或15%的FBS都可降低胚胎在体外的发育率,其体外囊胚发育率与对照组mM16(未加血清组)差异显著(73.3%对32.6%;73.3%对35.8%);5001、0001、500 IU/ml 3种不同浓度的hLIF都可提高小鼠受精卵体外发育率,三者之间差异不显著,但以添加1 000 IU/ml hLIF组中,桑椹胚/囊胚率及囊胚脱出率为最高。结论:在mM16中添加血清不能有效地支持小鼠受精卵在体外的发育,添加1 000 IU/ml hLIF能够很好地支持小鼠受精卵的体外发育。

The objective of this study was to probe into some culture conditions of mouse pronuclear zygote and establish a kind of steady culture system of mouse pronuclear zygote in vitro through the appeuding of the FBS and hLIF of different concantration in mM16. The ultama result indicated in raM16, the addition of 10% or 15% FBS could decrease the viability of zygote in vitro. The ratio of blastocyst formation to experimental and control groups were different（73.3% vs 32.6% and 73.3% vs 35.8%）, respectively; the different concentrations of hLIF （500, 1 000 and 1 500 IU/ml） can increase the development ratio of mouse zygote in vitro, which there were no obvious difference among of three groups. But this group added with 1 000 IU/ml hLIF, the ratio of momla, blastocyst and prolapsing blastocyst from the zona pellucida were the highest compared with other groups. The conclusion was the additionof FBS did not effectively accelerate the viability of mouse zygote in vitro, the appending of 1 000 IU/ml hLIF can rernarkably increase development ratio of mouse zygote in vitro.