摘要
本文采用高效液相色谱(HPLC)内标法测定血浆中比索洛尔浓度。色谱柱为TMS(7μm,0.46cm×15cm);流动相为乙腈-磷酸二氢胺(1mol/L,pH4)-水(18:5:77),荧光检测波长275/305nm(Ex/Em)。血浆样品经碱化二氯甲烷提取,催醒胺作内标。方法线性范围为2~50ng/ml;检测下限2ng/ml;在5、15和50ng/ml3种浓度平均回收率为(88.1±6.2)%;日内日间RSD均小于8.0%。
A HPLC method using fluorimetric detection was described for the quantitative determination of bisoprolol in plasma. The analytical column was packed with TMS of particle size 5μm.The mobile phase consisted of a mixture of acetonitrile : aqueous ammonium phosphate buffer (lmol/L, pH4) : H_2O (18 : 5 : 77). The detect wavelength was 275/305 nm (Ex/Em). Thebisoprolol in plasma were extracted at alkaline with dichloromethane. The extract were chromatographed on HPLC system after evaporation of the organic solvent. The calibration curve was linear within the range of 2~50 ng/ml plasma and the detection limit was 2 ng/ml. The mean recovery in samples of 5、15、 50 ng/ml was (88. 10±6. 15)% and the RSD for the inter and intra-day were all less than 8%.
出处
《药物分析杂志》
CAS
CSCD
北大核心
1996年第2期78-80,共3页
Chinese Journal of Pharmaceutical Analysis