新城疫病毒瘤苗对荷瘤小鼠的主动免疫治疗作用

Antitumor efficacy of active immunotherapy with Newcasle disease virus modified tumor cell Vaccine for Mice Loading primary liver cancer

将小鼠原发性肝癌细胞（Ｈ２２）经γ射线照射灭活后，与新城疫病毒（ＮＤＶ）共同孵育，制备病毒瘤苗，透射电镜下见ＮＤＶ吸附于Ｈ２２细胞表面。以病毒瘤苗皮内注射治疗不同负荷的Ｈ２２肿瘤荷瘤小鼠，连续２次，间隔１周，每次每只小鼠免疫剂量为１．５×１０７个肿瘤细胞。结果表明病毒瘤苗能抑制肿瘤生长，对荷瘤小鼠有主动特异性免疫治疗作用。各免疫组小鼠存活时间较对照组明显延长，肿瘤重量较对照组轻，肿瘤的平均直径也较对照组小，免疫组荷瘤存活小鼠肿瘤病理切片显示肿瘤细胞呈中、重度坏死，坏死的肿瘤细胞之间及其周围可见大量淋巴细胞及单核样细胞浸润，并有纤维组织增生。

The irradiated tumor cells of primary liver cancer H22were incubated with low dose Newcastle disease virus in order to obtain virus modified tumor cell vaccine.Transmission electron microscopy showed that Newcastle disease virus adhere to tumor cell membrane.Vaccines were injected intradermally into mice with transplanting H22 tumor in a dosage of 1.5×107 tumor cells,two times succesively with an interval of a week.Results showed that the active specific immunotherapy with this vaccine can inhibit the growth of the tumor.The mean survival time of immunotherapy groups is much longer than that of the control group.The weight and mean diameter of tumor mass has significant difference from that of the control group.The histopathology shows that there are a large number of tumor infiltrating lymphocytes and macrophage in tumor mass of immunized group.