类风湿关节炎急性期患者血清中瘦素及相关因子水平的变化

Serum leptin level increases significantly in acute rheumatoid arthritis

目的检测类风湿关节炎(RA)急性期患者血清中瘦素(leptin)及相关因子水平的变化,探讨Leptin在RA急性期发生过程中可能存在的意义。方法用高灵敏的放射免疫分析法及酶联免疫吸附试验(ELISA)测定RA患者血清中Leptin、白细胞介素(IL)-1β、IL-2及C反应蛋白(CRP)的水平。结果①RA组男性[(3．84±0．50)ng/ml]与正常对照组中男性[(0．89±0．13)ng/ml]相比,血清Leptin水平显著升高(P＜0．05)、RA组女性[(9．77±0．52)ng/ml]与正常对照组中女性[(1．78±0．26)ng/ml]相比,血清Leptin水平显著升高(P＜0．05)；②RA组患者与正常对照组相比,血清IL-1β、IL-2及CRP水平均显著升高(P＜0．05)；③经相关性分析发现各组Leptin水平的变化与IL-1β、IL-2及CRP水平的变化均无显著相关性。结论提示Leptin可能作为一种独立的炎症应答因子,在RA急性期发生过程中发挥重要作用。

Objective To detect serum levels of leptin and some related cytokines in rheumatoid arthritis patients, and to explore the possible role of leptin in acute rheumatoid arthritis. Methods We used sensitive radioimmunoassays for leptin, interleukin-1β （IL-1β）, and interleukin-2 （IL-2） measurement, and enzyme-linked immunosorbant assay （ELISA） for C-reactive protein （CRP） examination. The serum levels of above cytokines in rheumatoid arthritis patients were detected. Results （1）Compared with leptin levels of the normal male individuals （0.89±0.13） ng/ml, the leptin levels of the male rheumatoid arthritis patients increased significantly （3.84±0.50） ng/ml （P〈0.05）. Compared with leptin levels of the normal female individuals （1.78±0.26） ng/ml, the leptin levels of the female rheumatoid arthritis patients increased significantly （9.77±0.52） ng/ml （P〈0.05）; （2） Compared with normal individuals, IL-1±, IL-2 and CRP levels increased significantly in rheumatoid arthritis patients （P〈0.05）; （3） There was no correlation between serum levels of leptin and IL-1β, IL-2 or CRP. Conclusion Leptin, as an independent inflammatory factor, may have an important role in acute rheumatoid arthritis.