颞下颌关节骨关节病髁突软骨细胞线粒体DNA突变的实验研究

Analysis of the mitochondrial mutations of articular chondrocyte in temporomandibular joint osteoarthrosis

目的:探讨颞下颌关节骨关节病髁突软骨细胞线粒体DNA突变及其意义。方法:去除左侧部分关节盘建立大鼠颞下颌关节骨关节病模型,培养骨关节病(术后3月)和正常髁突软骨细胞。采用32对引物,使用PCR技术部分重叠扩增全长线粒体DNA,并将PCR产物进行时间温度梯度电泳,对电泳条带与正常有差异的PCR产物进行测序。结果:在35个具有异质性突变特点的PCR产物中,发现42个异质性突变,tRNA和Dloop具有高突变率。结论:颞下颌关节骨关节病髁突软骨细胞线粒体DNA存在突变。

Objective ：To explore the significance of mitochondrial DNA mutations of articular chondrocytes in the rat model of temporomandibular joint osteoarthrosis （TMJOA）. Methods：TMJOA models were created in left sides of TMJ of 15 SD rats by the partial resection of the articular disc. The experimental rats were killed 3 months after operation. After the chondrocytes culture, the entire mtDNA were amplified using 32 pairs of overlapping. DNA fragments showing different banding patterns between normal and experimental mtDNA by temporal temperature gradient gel electrophoresis were sequenced to identify the mutations. Results： Of the 35 heteroplasmic pattern PCR products, 42 novel mutations were found, Majority of the novel mutations were in the tRNA and D-loop regions. Conclusion：The mutations occurred in the mtDNA of TMJOA articular chondrocytes.