预适应对氧化应激中HepG2细胞保护作用的初步探讨

Role of preconditioning to oxidative stress in HepG2 cells

目的:建立HepG2细胞预适应、氧化应激模型。方法:应用不同浓度H2O2作用于HepG2细胞,分别用吖啶橙和溴化乙锭(AO/EB)双染色法、MTT比色法及PI染色流式细胞术检测细胞活力及凋亡情况。结果:各组细胞经AO/EB双染色之后呈现不同的染色状态:对照组细胞为正常梭形,呈均匀绿色荧光染色;预适应组可见少量绿色浓染细胞;氧化应激组可见大量绿色或红色浓染凋亡细胞;预适应后氧化应激组凋亡细胞明显少于氧化应激组。预适应组MTT比色法测定细胞生存活力比较:对照组>预适应组>预适应后氧化应激组>氧化应激组(P<0·05)。PI染色流式细胞术测细胞凋亡率比较:氧化应激组>预适应后氧化应激组>预适应组>对照组(P<0·05)。结论:不同浓度H2O2作用于HepG2细胞发生预适应和氧化应激现象,应用小剂量H2O2作用于细胞可以保护细胞免受更大浓度H2O2带来的损伤。

AIM： To induce preconditioning and oxidative stress by H2O2 in HepG2 cells. METHODS： The different doses of H2O2 were used to induce apoptosis in HepG2 cells, which was estimated by AO/EB staining, MTT assay and flow eytometry. RESULTS：The different group of HepG2 cells stained with AO/EB showed different staining state. The high dose of H2O2 resuited in the increase in apoptosis rate of HepG2 cells and made MTT activity decreased. However, after pretreated with low dose of H2O2, the apoptosis rate was decreased and MTT activity was increased. CONCLUSION： The high dose H2O2 induced apoptosis in HepG2 cells and the low dose H2O2 protected HepG2 cells against the oxidative stress.