两组不同月龄小鼠学习记忆能力与脑突触体内游离［Ca^（2＋）］_i的相关性

CORRLEATION BETWEEN ABILITY OF LEARNINGMEMORY AND SYNAPTOSOMAL FREE[Ca￣(2+)]_ i IN MICE OF DIFFERENT AGE

本文采用Ｙ－迷宫和一次被动回避反应模型，观察了１，６月龄小鼠的学习记忆行为，并运用Ｃａ^（２＋）的荧光探针和ＡＲ－ＣＭ－ＭＩＣ阳离子测定系统，检测了两组小鼠四个主要脑区（海马、皮层、小脑、中脑四叠体）突触体内游离钙离子浓度（［Ｃａ^（２＋）］_ｉ）的变化。结果表明随着年龄的增长，小鼠的分辨学习及记忆能力均下降，同时海马突触体内游离［Ｃａ^（２＋）］_ｉ升高显著。

In the present investigation, the behavior of learning and memory of 1-month and6- month-old mice was studied by using Y-maze and one-trial passive avoidance response device. The synaptosomal free [Ca￣(2+)]_i of four main brain regions (Hippocampus, Cerebral cortex, Cerebellum, Tectum of midbrain) of these mice were measuredby fluorescent probe Ca￣(2+) indicator Fura-2 and an AR-CM-MIC cation measurementsystem. The results showed that, in comparison with 1-month-old mice, the ability ofdiscrimination learning and memory of 6-month-old ones were attenuated, and thesynaptosomal flee [Ca￣(2+)]_i of hippocampus was increased.