福建山樱花不定芽诱导和植株再生规模化繁殖试验

Adventitious Bud Inducing and Plant Regeneration of Cerasus campanulata Maxim.in Large Scale

取长约4 cm的3年生福建山樱花中选树的春梢为外植体,接种于添加6-BA和IBA的MS培养基中,诱导产生不定芽。结果表明:当培养基为1/4 MS时,能有效地防止外植体的褐化死亡;添加1.0 mg/L 6-BA和0.01 mg/L IBA的培养基上,外植体能快速生长,且无玻璃化苗发生。增殖阶段以添加1.0 mg/L 6-BA+0.3 mg/L GA3的MS培养基最好,1个周期的增殖率可达600%。当外殖体高约4 cm时,即转入1/2MS+1.0 mg/L NAA+0.2 mg/L IBA培养基进行生根诱导培养,25 d后即可陆续生根并长成完整植株,35 d生根率可达92.1%。以1/3份珍珠岩+2/3份泥炭混合基质为移栽基质时,试管苗移栽成活率可达94%。该试验中各项指标已达到快速繁育苗木的要求,其技术措施可直接应用于规模化生产。

The explants in 4 cm length collected from spring shoots of 3-year-old selected tree of Cerasus campanulata were inoculated on MS culture medium with 6-BA and IBA for adventitious buds mutiplication. The results showed that explants were prevented effectively from being brown and dying when the medium of 1/4MS were used. Explants grew fast and no vitrification when the medium plused with 1.0 mg/L 6-BA and 0.01 mg/L IBA. The medium of MS with 1.0 mg/L 6-BA and 0.3 mg/L GA3 was the best for multiplication and multiplication rate could reach to 600 % during one culture cycle. Plantlets were to be transferred on the 1/2MS medium with 1.0 mg/L NAA and 0.2 mg/L IBA for rooting, when the explants were reached in about 4 cm. The root system could be developed well during 25 days and to be come a new plant. The rooting rate could reach to 92.1% after 35 days. The survival rate was above 94% when the plantlets were to be moved to nursery. The medium in 34% pearlite and 67% peat soil was good for rooting. This micropropagation technology could be applied in mass propagation.