烟草柱头和花柱中阿拉伯半乳糖蛋白的定位

The Localization of Arabinogalactan-Proteins in Stigma and Style of Nicotiana tabacum L.

通过Western印迹法、免疫组织化学和超微细胞化学等技术,研究了烟草柱头和花柱中阿拉伯半乳糖蛋白(arabinogalactan-proteins,AGPs)的分布。结果表明烟草柱头和花柱组织中含有大量的AGPs,主要分布于柱头表皮细胞的细胞质和分泌层细胞的胞外基质中,且授粉前后AGPs的分布情况差异不明显;而花柱中的AGPs主要分布于表皮细胞的外层细胞壁、维管组织周围细胞的细胞质及引导组织的胞外基质中;花粉管通过后,引导组织胞外基质中AGPs减少,而花粉管细胞质和花粉管壁中检测到大量AGPs。

The distribution patterns of arabinogalactan-proteins （AGPs） in stigma and style of Nicotiana tabacum L. were studied with western blotting, immunohistochemistry and ultracytochemistry techniques. The results showed abundant AGPs accumulated in the stigmas and styles （Fig. 1, Plate Ⅰ）. AGPs were mainly distributed in the cytoplasm of epidermal cells and extracellular matrix of the secretory zone of stigma （Plate Ⅱ） and no significant changes of the distribution of AGPs in stigma were detected before and after pollination. In styles, AGPs were mainly distributed in the outer cell walls of the epidermal cells, the cytoplasm of peripheral cells of vascular bundles and the extracellular matrix of the transmitting tissues （Hate Ⅲ）. As the pollen tube tips penetrated through, the accumulations of AGPs in the extracellular matrix of the stylar transmitting tissues decreased, but abundant AGPs were founded in the cytoplasm and cell walls of the passing pollen tubes （Hate Ⅲ）.