bcl-2基因短发夹RNA对自体移植静脉内膜增生的影响

Effect of bcl-2-shRNA on the intimal hyperplasia of vein autografts

目的探讨针对bcl-2基因短发夹RNA对兔自体移植静脉内膜增生的影响。方法18只新西兰白兔,取颈外静脉,用含pshRNA-bcl-2质粒(基因组)或pH1质粒(空载体组)的阳离子脂质体溶液浸泡,空白对照组无特殊处理,间置于同侧颈总动脉。术后4周采用半定量逆转录-聚合酶链反应(RT-PCR)和Western blot检测bcl-2基因的表达,病理形态学观察血管新内膜增生改变。结果基因组移植静脉内膜的bcl-2的mRNA和蛋白均明显受到抑制。组织学检查显示,基因组新内膜厚度为(85．78±5．47)μm,空载体组为(175．2±10．90)μm,差异有统计学意义(P＜0．01)。结论bcl-2基因的短发夹RNA可能通过降低血管bcl-2蛋白的表达抑制移植静脉内膜增生。

Objective To investigate the inhibitory effect of shRNA targeting bcl-2 on intimal hyperplasia of vein autugrafts. Methods Eighteen New Zealand white rabbits underwent implantation of reversed autolugous jugular vein interposition grafts in the common carotid artery. Jugular veins were exercised and placed in solution containing either pshRNA-bcl-2 （gene therapy group） or pill （vector control group） for 30 min. In in the normal control group, no medication was applied. Then the jugular veins were reversed and interposed into the divided carotid arteries. The vein autografts were removed and measured by means of pathology and RT-PCR and Western blot 4 weeks later. Results The bcl-2 mRNA and protein expression index were obviously inhibited in the gene therapy group as compared with that in the other two control groups （P〈0.01）. The thickness of neointima in the gene therapy group was （ 85.78 ± 5.47 ） μm, and （ 175.2 ± 10.90） μm in the vector control group （ P〈0.01 ）. Conclusion The plasmid containing the shRNA of bcl-2 could effectively inhibit intimal hyperplasia of rat vein autografts, which was contributed to the decrease of the expression of bcl-2 protein in intima.