高效液相色谱法测定脑得生片中葛根素的含量

Determination of puerarin in Naodesheng tablets by HPLC

目的采用高效液相色谱法测定脑得生片中葛根素的含量，以控制该制剂的质量。方法柱为Agilent EclipseXDB—C18（4．6×250mm，51μm）；以水-甲醇-乙腈溶液（76：20：4）为流动相；流速1．0mL／min；检测波长为250nm。结果方法的平均加样回收率为102．0％，RSD=0．62％（n=9）。葛根素在0．032-2．03μg进样量范围内，进样量与吸收面积值呈良好的线性关系R=1．000。结论本方法测定脑得生片中葛根素的含量，结果准确,重复性好。

OBJECTIVE To determine the contents of puerarin in Naodesheng tablets by HPLC. METHODS Agilent Eclipse XDB-C18 column （4.6 × 250mm, 5 μm） was used with the mobile phase of water-methanol-acetonitrile （ 76： 20： 4） , the flow rate was 1.0mL/min. The wavelength of detection was set at 250nm and the column temperature was at 25℃. RESULTS The linear range of puerarin was 0. 032 - 2. 031μg with the correlation coefficient 1. 000. The average recovery was 102.0%. CONCLUSION The method is simple,rapid and accurate.