新型人趋化素样因子超家族成员8对肿瘤细胞增殖和EGFR表达的影响

Effect of novel human chemokine-like factor superfamily 8 on proliferation and EGFR expression of tumor cells

目的:研究新型细胞因子CKLFSF8对肿瘤细胞增殖及EGFR表达的作用。方法:首先利用RT-PCR了解CK-LFSF8基因在肿瘤细胞株BGC823和K562中的表达,然后在脂质体介导下将CKLFSF8基因转染这两种肿瘤细胞,倒置显微镜观察细胞生长,MTT法检测细胞增殖,免疫细胞化学法检测肿瘤细胞中EGFR的表达。结果:两种肿瘤细胞生长过程中均可测到CKLFSF8基因的表达。转染CKLFSF8后对BGC823和K562的增殖均有抑制作用,与空白对照组和空质粒组有统计学意义(P<0.05);CKLFSF8转染前后BGC823和K562细胞EGFR阳性率对比有统计学意义(P<0.05)。结论:CKLFSF8基因转染对肿瘤细胞的增殖和EGFR的表达有明显抑制作用,可望在肿瘤治疗中发挥重要作用。

AIM： To investigate the effect of chemokine-like factor superfamily 8 （CKLFSF8） on proliferation and epidermal growth factor receptor （EGFR） expression of tumor cells. METHODS： Expression of CKLFSF8 mRNA in BGC823 and K562 cells was assayed by RT-PCR. Then target gene was transfected into tumor cells by lipid method, and cell growth was observed by inverted microscope. Cell proliferation was determined by MTT assay and expression of EGFR on tumor cells was determined by immunocytochemical technique. RESULTS： CKLFSF8 was expressed in the two tumor cells. After transfection, cell proliferation was inhibited （ P 〈 0. 05 ） and the expression of EGFR of tumor cells was also discovered to be inhibited （ P 〈0.05 ）. CONCLUSION： The finding shows that cell proliferation and expression of EGFR of tumor cells can be inhibited by transfection of CKLFSFS. The new chemokine may has a bright prospect in the treatment of tumor.