期刊文献+

温肾中药对原代培养正常人乳腺上皮细胞增殖的影响 被引量:10

Effects of Chinese herbal medicine for warming kidney on proliferation of normal human mammary epithelial cells in primary culture
下载PDF
导出
摘要 目的观察温肾中药对雌二醇和孕酮所致的原代培养正常人乳腺上皮细胞增殖的影响。方法采用消化法进行正常人乳腺上皮细胞分离及原代培养。在相差倒置显微镜和透射电镜下对细胞进行形态学鉴定。应用雌二醇和孕酮干预以促使正常人乳腺上皮细胞增殖,给予不同浓度温肾中药及他莫昔芬进行药物干预。采用MTT法检测不同浓度、不同药物干预下乳腺上皮细胞的增殖情况。结果原代培养正常人乳腺上皮细胞经形态学鉴定符合正常人乳腺上皮细胞的形态学特征。浓度均为1×10-5g/L的雌二醇和孕酮混合培养液可以明显促进正常人乳腺上皮细胞增殖。高浓度温肾中药能明显促进原代培养正常人乳腺上皮细胞增殖,而低浓度温肾中药则可抑制其增殖。他莫昔芬对原代培养正常人乳腺上皮细胞增殖无明显影响。结论雌二醇和孕酮可以促进正常人乳腺上皮细胞增殖。温肾中药调节正常人乳腺上皮细胞增殖的作用与药物浓度相关。 Objective: To evaluate the effects of Chinese herbal medicine for warming kidney on proliferation of normal human mammary epithelial cells in primary culture. Methods: The normal human mammary epithelial cells were dissociated by digestion with collagenase type Ⅰ The morphological identification of normal human mammary epithelial cells in primary culture was determined under inverted phase contrast microscope and transmission electron microscope. The estradiol and progesterone were added to culture medium to induce the proliferation of normal human mammary epithelial cells in primary culture, and the different doses of Chinese herbal medicine for warming kidney and tamoxifen were also added into the culture medium. The MTT assay was used to evaluate the proliferation of normal human mammary epithelial cells in primary culture under the different conditions of interventions. Results: The normal human mammary epithelial cells in primary culture presented typically morphological features of normal human mammary epithelial cells in vitro. The proliferation of normal human mammary epithelial cells in primary culture was significantly improved by intervention of estradiol and progesterone both with concentration of 1 ×10^-5 g/L. The high-dose Chinese herbal medicine for warming kidney could obviously promote the proliferation of normal human mammary epithelial cells in primary culture, while the proliferation was obviously inhibited by low-dose Chinese herbal medicine for warming kidney. The tamoxifen exerted no effects on the proliferation of normal human mammary epithelial cells in primary culture. Conclusion: The estradiol and progesterone can enhance the ability of proliferation of normal human mammary epithelial cells in primary culture. The regulating effect of Chinese herbal medicine for warming kidney on proliferation of normal human mammary epithelial cells in primary culture is dose-dependent.
出处 《中西医结合学报》 CAS 2006年第4期397-401,共5页 Journal of Chinese Integrative Medicine
基金 上海市重点学科建设资助项目(No.T0304)
关键词 温肾 中草药 原代培养 乳腺 上皮细胞 细胞增殖 warming kidney drugs, Chinese herbal primary culture mammary glands, human epithelial cell cell proliferation
  • 引文网络
  • 相关文献

参考文献15

  • 1Freshney RI,Pragnell IB,Freshney MG.Culture of epithelial cells.Second edition.New York:Wiley-Liss,2002.3.
  • 2胡润淮,裴广战.RP-HPLC法测定复方仙灵脾注射液中淫羊藿苷含量[J].中成药,2002,24(2):97-99. 被引量:12
  • 3Raafat AM,Hofseth L J,Haslam SZ.Proliferative effects of combination estrogen and progesterone re placement therapy on the normal postmenopausal mammary gland in a murine model.Am J Obstet Gynecol,2001,184(3):340-349.
  • 4Persson I,Thurfjell E,Holmberg L.Effect of estrogen and estrogen-progestin replacement regimens on mam mographic breast parenchymal density.J Clin Oncol,1997,15(10):3201-3207.
  • 5Greendale GA,Reboussin BA,Sie A,et al.Effects of estrogen and estrogen progestin on mammographic parenchymal density.Postmenopausal Estrogen/Progestin Interventions (PEPI) Investigators.Ann Intern Med,1999,130(4 Pt 1):262 269.
  • 6陈红风.“乳块消”治疗乳腺增生病的实验研究[J].上海中医学院上海市中药研究院学报,1993,7(2):48-52.
  • 7唐栩,许东晖,梅雪婷,许实波.26种黄酮类天然活性成分的药理研究进展[J].中药材,2003,26(1):46-54. 被引量:75
  • 8李炳如 佘运初.补肾药对下丘脑-垂体-性腺轴功能的影响[J].中医杂志,1984,25(7):63-63.
  • 9Liu J,Ye H,Lou Y.Determination of rat urinary metabolites of icariin in vivo and estrogenic activities of its metabolites on MCF-7 cells.Pharmazie,2005,60(2):120-125.
  • 10陈泉生,陈万群,杨士琰.仙茅的药理研究[J].中国中药杂志,1989,14(10):42-44. 被引量:27

二级参考文献107

共引文献217

同被引文献130

引证文献10

二级引证文献62

;
使用帮助 返回顶部