RP-HPLC测定乳癖消片中三七皂苷R_1和人参皂苷Rg_1的含量

Determination of notoginsenoside R_1 and ginsenoside Rg_1 in Rupixiao tablets by HPLC

目的：应用RP-HPLC测定乳癖消片中三七皂苷R1和人参皂苷Rg1的含量。方法：以乙腈-0．05％磷酸溶液（20：80）为流动相，采用Kromasil C18柱，在203nm波长进行测定。结果：三七皂苷R1在0．941～9．41μg、人参皂苷硒在1．043～10．43μg线性关系良好，平均回收率分别为97．3％，97．9％。结论：该方法简便、准确、可靠，可以用于该制剂的质量控制。

Objective： To determine the content of notoginsenaside R1 and ginsenoside Rg1 in Rupixiao tablet by reverse-phase high performance liquid chromatography. Method： A Kromacil C18 column was used with a mixture of aeetonitrile and 0.05% phosphoric acid solution （20：80） as the mobile phase at a flow rate of 1.2 mL·min^-1 with the detection wavelength at 203 nm. Result： The measmurement proved to be linear over the range of 0.941-9.41 g for notoginsenaside R1 and 1.04-10.4 g for ginsenaside Rg1. The average recovery of this method was 97.3% and 97.9% respectively. Conclusion： The method was simple, reliable, and accurate and can be used for the quality control of this preparation.