国产虫草素(cordycepin)抗小鼠迟发型超敏反应的实验研究

Effect of cordycepin on inhibiting delayed type hypersensitivity

目的:研究国产虫草素抗小鼠迟发型超敏反应的作用及其免疫机理。方法:用2,4-二硝基氯苯(2,4-DNCB)对昆明种小鼠皮肤致敏和诱发,制成迟发型超敏反应模型。以国产虫草素溶液作为实验组,分为两个剂量组(实验组1:12mg/kg;实验组2:16mg/kg),以生理盐水作为阴性对照组,所有溶液均为间隔24小时(最后一次间隔为4小时)重复腹腔注射给药。计算激发后各组小鼠左右耳耳廓肿胀厚度差、耳廓增重值及肿胀度,进行统计分析,并经过HE染色观察虫草素对小鼠致炎耳炎症病理变化及脾脏病理变化的影响。结果:两种给药剂量的国产虫草素溶液对模型鼠红斑,水肿,渗出的接触性皮炎损害有明显抑制作用,可减轻由于充血、水肿等炎症反应导致的局部皮损增厚及重量的增加。与生理盐水对照组相比,两实验组虫草素溶液的抗炎作用均有显著性差异(厚度差:P<0·0001;肿胀度:P<0·05);两实验组间的抗炎作用也有显著性差异(厚度差及肿胀度均为P<0·05),且与给药剂量相关。三组小鼠脾指数未见明显差异(P>0·05),脾脏组织病理变化未见明显差异。结论:虫草素以24小时间隔(最后一次给药间隔为4小时)经腹腔注射后,可能通过其免疫调节作用对迟发型超敏反应引起的小鼠接触性皮炎发挥明显的抑制效应,该效应与给药剂量相关,同时对脾脏组织未见明显毒性作用。

Objective：To investigate the effect and the mechanism of cordycepin on inhibiting delayed type hypersensitivity. Methods：The mouse model of delayed type hypersensitivity was established by sensitizing the skin of Kunming mice with 2,4-dinitrochlorobenzen（2,4-DNCB）. There are two experimental groups respectively treated with different dosages of cordycepin：12 mg/kg and 16 mg/kg and one negative control group treated with normal saline. All the mice were intraperitoneally injected at 24 h interval（ the last interval was 4 hours）. We calculated the differences of the thickness and the weight between the left and right earlaps of every mouse and the edema rate, then used statistical methods to compare the edema rate and the spleen index among all the groups. After HE staining, the pathological changes of inflammatory earlap and spleen were observed and compared among these three groups. Results：Cordycepin alleviated the erythema, edema and the infiltration of peripheral mononuclear cells in the earlaps, so lowering the differences of the thickness and weight between left and right earlaps in the experimental groups. But it showed no significant effects on the spleen index and the spleen pathological changes. Compared with that in the negative control group, the anti-inflammatory effects of cordycepin in two experimental groups showed significant differences （ P 〈 0. 05 ） and were correlated with dosage. Even between two experimental groups, the result was the same（P 〈0. 05 ）. Conclusion：Cordycepin can markedly inhibit the delayed type hypersensitivity with dosage-dependence after intraperitoneal injection at 24 h interval（ the last interval was 4 hours） possibly by its immunoregulative function.