河南省新疆出血热血清流行病学调查

Seroepidemiological Investigation on CCHF in Henan Province

目的调查河南省新疆出血热的感染情况。方法采集不同地区牧羊人、一般农民、发热病人、既往EHF患者及家属的人血清标本及动物血清标本作横断面调查,采用基于昆虫细胞表达的CCHFV核蛋白建立的ELISA法对血清标本进行初筛,阳性及可疑阳性再用表达CCHFV核蛋白的HeLa细胞进行IFA试验加以确证。结果254份人血清及242份羊血清标本全部为阴性。结论调查表明河南省尚未发现新疆出血热病毒感染。应进一步调查,加强监测,做好防范新疆出血热的准备,尤其预防新疆出血热的输入感染。

Objective To investigate the attack rate of CCHF in Henan province. Methods Sere samples from shepherds, veterinarians, slaughter workers, fever cases and animal hosts of CCHFV were collected in different areas of Henan province, positive samples were screened primarily by ELISA based on recombinant Baculovirus expressed CCHFV- NP and then confirmed by IFA which was based on CCHFC - NP expressing HeLa cells. Results All sere samples were negative for CCHF virus antibody. Conclusion The investigation results showed that no CCHFV infection was found in both human and animals in Henan province, This suggested that the surveillance of CCHF should be strengthened, and necessary measures should be adopted to prevent the possible occurrence of CCHF.