三叶青对CCl_4致肝损伤大鼠血清五项生化指标水平的影响

Effects of Tetrastigmatis Hemsleyani on the levels of five serous biochemistry indexes in hepatic injury rats induced by CCl_4

目的:研究三叶青水煎液对CC l4致大鼠急性肝损伤的保护作用。方法:将40只SD大鼠随机分为5组,即正常对照组,模型对照组,高、低剂量治疗组及阳性对照组,分别灌胃等容积(10 mL/kg)的生理盐水、三叶青水煎液(1.6 g/mL、0.16 g/mL)和联苯双酯(0.000208 g/mL),连续6 d,每天1次。末次给药后,除正常组外其他各组腹腔注射CC l4原液1次,造成大鼠急性肝损伤模型,检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)、丙二醛(MDA)及超氧化物歧化酶(SOD)水平。结果:与模型组比较,三叶青高、低剂量治疗组ALT、AST、ALP及MDA含量均明显降低(P<0.05或P<0.01),但SOD活性明显升高(P<0.05或P<0.01);高剂量组与阳性对照组比较,AST、ALP及MDA水平差异显著(P<0.05)。结论:三叶青对CC l4造成的大鼠急性肝损伤具有保护作用,且三叶青高剂量保护损伤肝的效果优于联苯双酯。

Objective： To research the protective effects of water extract of Tetrastigmatis Hemsleyani （WETH） on acute hepatic injury induced by CCl4 in rats. Metheds：40 SD rats were randomly divided into five groups, namely the normal control group, the model control group, the high and low dose treating group and the positive control group. They were intragastricaUy administrated with NS, WETH （ 1. 6 g/mL and 0. 16 g/mL） and bifendate（0.000208 g/mL） at the equal volume dose（ 10 mL/kg） once daily for six days. After the last administration, all groups except the normal group were intraperitoneally injected with CCl4 stock solution to induce acute hepatic injury model. Five serous biochemistry indexes were detected, including ALT, AST, ALP, MDA and SOD. Besults： Compared will the model group, the contents of ALT, AST, ALP and MDA remarkably decreased （ P 〈 0.05 or P 〈 0.01 ）, but the activity of SOD remarkably improved （P 〈 0. 05 or P 〈0.01 ） after administration with high and low dose of WETH. The contents of AST, ALP and MDA in high dose treating group were prominently lower than that in positive control group（ P 〈0.05）. Conclusion： WETH has protective effects on acute hepatic injury of rats induced by CCl4, the protective effects of high dose of WETH surpass the low dose.