O-羧甲基乳糖酰化壳聚糖-聚乳酸阿霉素纳米粒在大鼠体内的靶向性研究

Research on targeting of O-Carboxymethylated Chitosan-PLA Adriamycin nanoparticles in rats

目的探讨O-羧甲基乳糖酰化壳聚糖-聚乳酸阿霉素纳米粒在大鼠体内的靶向性。方法全部大鼠随机分为4组,每组30只,A组:游离阿霉素组;B组:O-羧甲基壳聚糖聚乳酸阿霉素组;C组:壳聚糖-聚乳酸阿霉素纳米粒组;D组:O-羧甲基乳糖酰化壳聚糖-聚乳酸阿霉素纳米粒组;经舌静脉,按分组分别注射;取全血、心、肺、肝、脾、肾。全血制成血浆,器官组织制成匀浆,盐酸乙醇法提取阿霉素,用荧光光度计测量。结果静脉注射同等剂量、不同剂型的阿霉素药物后,半乳糖化修饰后纳米粒的肝靶向性明显增强,肝外器官的浓度明显降低。结论O-羧甲基乳糖酰化壳聚糖-聚乳酸阿霉素纳米粒对正常大鼠肝脏具有明显的靶向性。

[Objective] Discuss the targeting of O-Carboxymethylated Chitosan -PLA Adriamycin nanoparficles in rats. [Methods] All animals were divided into four groups. The drugs were administrated tissues which were rinsed for homogenate. Adriamycin was picked up by hydrochlore-ethanol method, whose level was measured by fluorescence photometer. [Results] The concentration of liver increase significantly, The concentration of heart, kidney, spleen, small intestine and blood in experiment group was descreased significantly as compared to control. [Conclusion] O-Carboxymethylated Chitosan-PLA Adriamycin nanoparficles had remarkable hepatic targeting in normal rat liver.