大黄蟅虫丸微生物限度检查法的建立

Establishment of Microbial Limit Test for Dahuang Zhechong Pill

目的建立大黄蟅虫丸微生物限度检查法。方法测定大黄蟅虫丸对大肠埃希菌等5种试验菌株回收率,并对3个控制菌的检查法进行验证。结果大黄蟅虫丸对金黄色葡萄球菌及枯草芽孢杆菌有较强的抑制作用,对大肠埃希菌的抑制作用很微弱;而对白色念珠菌和黑曲霉菌几乎无抑制作用。结论采用离心沉淀法与培养基稀释法相结合,可消除大黄蟅虫丸在试验条件下的抑菌作用,从而顺利检出该品种所污染的各种微生物。

objective Microbial limit test （MLT） for Dahuang Zhechong pill was established and verified to supply scientific foundation for the establishment of MLT for antibacterial and anti -inflammatory Chinese patent medicine, and to supply principle guidance for the verification of the method issued in Chinese pharmacopoeia （2005 edition）; meanwhile the antibiogram of Dahuang Zhechong pill was probed, which can provide evidence for its clinical administration. Methods With centrifugal sedimentation method and culture medium diluted method, the recovery test was done on each tested bacterial strain to validate the accuracy and feasibility of MLT. Results Dahuang Zhechong pill had a stronger inhibitory, effect on staphylococcus aureus and bacillus subtilis, had a weak inhibitory effect on escherichia coli, and had no effect on candida albicans and aspergillus niger, indicating that the antimicrobial spectrum of Dahuang Zhechong pill is Gram - positive bacteria. Conclusion Centrifugal sedimentation method combined with culture medium diluted method can eliminate the anti - bacterial effect of Dahuang Zhechong pill during the experiment, thus the microorganisms in the polluted pill can be detected successfully.