快速溶剂萃取反相高效液相色谱法测定青蒿中的青蒿素

Rapid pressurized solvent extraction and RP-HPLC determination of artemisinine in Artemisia Annua L.

报道了一种应用快速溶剂萃取、经衍生化处理后用RP-HPLC测定青蒿药材中青蒿素的方法。青蒿样品用无水乙醇萃取剂在90℃，12．6MPa压力下萃取10min，用碱衍生化后，色谱测定，回收率在95．3％-101．2％。色谱分析条件采用Diamonsil C18色谱柱（250mm×4．6mm，5μm），甲醇-0．02mol／L乙酸铵为流动相，梯度：甲醇体积分数20％-35％，5min；35％-98％，5min；98％，6min，检测波长260nm；流速：1．0mL／min；柱温：25℃。结果表明该法准确、重现性好，可以为青蒿质量标准的制订提供科学依据。

procedure tracted by A rapid pressurized-solvent extraction, followed a derivation reaction and reversed-phase HPLC analysis has been developed to determine the content of artemisinine in Artemisia annua L.. Artemisinine was exanhydrous ethanol for 10 min at temperature 90℃ and pressure 12.6 MPa. Extracts were derived by alkali and then analyzed by HPLC. The recovery was from 95.3% to 101.2%. A Diamonsil C18 column was used with a mixture of methanol - 0.02 mol/L ammonium acetate solution as the mobile phase, the detection wavelength is at 260 nm. The flow rate was set at 1.0 mL/min, and the column temperature was 25℃. The results showed that the method developed is accurate , rapid and repeatable.