摘要
应用3对人乳头瘤病毒(HPV)引物对107例各种宫颈标本进行了聚合酶链反应(PCR)扩增。结果显示,共同引物(GP)扩增,有58.8%(30/51)宫颈鳞癌,100%(14/14)尖锐湿疣、13.6%(3/22)宫颈炎和10%(2/20)正常宫颈出现HPV阳性。型特异性引物SP16/SP18扩增,有37.2%(19/51)宫颈鳞癌25.7%(5/14)尖锐湿疣和5%(1/20)正常宫颈出现HPV16型阳性,5.8%(3/51)宫颈鳞癌为HPV18型阳性。进一步用SP16b引物扩增,没有1例HPV16b亚型被发现。说明宜颈磷癌和尖锐湿疣与HPV感染有关,结合应用共同引物和型特异性引物可作为HPV检测与分型方法。
ThreepairsofprimersfromHPVgenomewereusedtoamplify107genitaltractspecimensfromnormalindividualsandpatientswithcervicitis,condylomaacuminatumorcervicalcarcinoma.Byuseofgeneralprimer(GP)selectedfromtheconservedL1ORF,thepresenceofHPVDNAwasdemonstratedin30of51(58.8%)cervicalcaccinoma,14of14(100%)condylomaacuminatum,3of22(13.6%)cervicitisand2of20(10%* )normalcervices.Usingtypespecificprimer(SP16/SP18)encodingtheURRofHPVgenome,wefoundthat19of51(37.2%)cervicalcarcinoma,5of14(35.7%)condylomaacuminatumand1of20(5%)normalcerviceswerepositiveforHPV16andthat3of51(5.8%)cervicalcarcinomawerepositiveforHPV18.Furthermore,whenSP16bprimerwasusedtoidentifythesubtypeofHPV16,HPV16bwasnotfoundinallspecimens.TheresultsindicatedthatHPVinfectionisassociatedwithcervicalcarcinomaandcondylomaacuminatum,andGPandSPmediated-PCRcan.thereforebeapowerfultoolforthedetectionandtypingofHPVinclinicalspecimens.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
1996年第3期229-232,共4页
Chinese Journal of Experimental and Clinical Virology
基金
本研究受江苏省卫生厅基金资助
关键词
人乳头瘤病毒
宫颈肿瘤
聚合酶链反应
DNA
病毒
CervicalneoplasmsDNA,viralCervicitisHumanpapillomavirusPolymerasechainreaction