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P物质对病理性瘢痕成纤维细胞增殖及凋亡影响的实验研究 被引量:3

Influence of substance P on the proliferation and apoptosis of fibroblasts of pathological scars
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摘要 目的探讨P物质(SP)对不同病理性瘢痕成纤维细胞(Fb)增殖及凋亡的影响。方法增生性瘢痕、瘢痕疙瘩及手术剩余正常皮肤标本取自笔者单位12例烧伤患者,采用组织块培养法体外培养Fb并传至6-8代。根据DMEM培养液中培养物质的不同分为SP组(含1×10-6 mol/L SP)、SP+SP受体拮抗剂(spantide)组(含1×10-6 mol/L SP、3×10-5 mol/L spantide)、空白对照组(含体积分数10%胎牛血清)。采用噻唑蓝比色法和流式细胞仪检测SP、spantide对瘢痕疙瘩Fb(KSF)、增生性瘢痕Fb(HSF)及正常真皮Fb(NDF)增殖活性[吸光度(A)值]及凋亡率的影响;另取1×10-6 mol/L SP分别培养Fb 24-120 h,以及SP组划分出1×10-9-1×10-5 mol/L的浓度梯度,检测SP对上述3组不同样本中Fb作用的时间、剂量效应。结果空白对照组各样本Fb的A值及凋亡率无明显差异。SP组KSF、HSF、NDF的A值分别为0.656±0.071、0.525±0.064、0.404±0.063,凋亡率分别为(1.5±0.3)%、(4.0±0.5)%、(5.5±0.7)%,与空白对照组比较,差异均有统计学意义(P< 0.05);SP对Fb作用的强弱为KSF>HSF>NDF。SP+spantide组部分抑制SP对KSF的作用而完全抑制其对HSF、NDF的作用。SP作用的时间、剂量效应检测结果显示,SP对KSF的作用较HSF、NDF更加灵敏、持久。结论SP对不同病理性瘢痕Fb增殖、凋亡的影响符合病理性瘢痕的临床特征,提示SP在其形成过程中发挥了重要作用。 Objective To further explore the effects of substance P on the proliferation and apoptosis of fibroblasts obtained from pathological scars in vitro. Methods Fibroblasts from keloid (KSF) , hypertrophic scar (HSF) and normal dermis (NDF) of 12 burn patients were cultured in vitro and divided into control, SP (with 1×10^-6mol/L SP added to the culture medium) , and SP + spantide(with 1×10^-6mol/L SP and 3×10^-5 mol/L spantide added to the culture medium)groups. MTT method or flow cytometry assay was used for the determination of the proliferative activities or apoptotic rate of fibroblasts obtained from KSF, HSF and NDF with SP or Spantide. And then the fibroblasts in SP group were subdivided into 1×10^-9 - 1×10^-5 mol/L groups to examine the time-or dose-effect of SP to fibroblasts from different sources. Results In control group, different types of fibroblasts exhibited similar proliferative activities and apoptotic rates. But there was significant difference in these indices between control and SP group (the proliferative activity of KSF, HSF, NDF was 0. 656 ± 0. 071, 0. 525 ± 0. 064, 0. 404 ± 0. 063, respectively; and the apoptotic rate ofKSF, HSF, NDFwas [(1.5±0.3) %, (4.0±0.5) %, (5.5±0.7) %, respectively],( P 〈 0. 05). SP had stronger effect on KSF than it did to HSF, as well as it had stronger effect on HSF than it did to NDF. In SP + spantide group, the effect of SP on KSF was partially inhibited, while it was completely inhibited in cultures of HSF and NDF. KSF was more sensitive to SP and the effect was longer when compared with HSF. Conclusion SP may play an important role in the process of pathological scar formation due to its diverse effects on fibroblasts from different sources.
出处 《中华烧伤杂志》 CAS CSCD 北大核心 2006年第4期277-280,共4页 Chinese Journal of Burns
关键词 P物质 成纤维细胞 细胞增殖 细胞凋亡 病理性瘢痕 Substance P Fibroblasts Cell proliferation Apoptosis Pathological scar
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