摘要
目的了解脱氧核糖核酸核蛋白体(rDNA)转录活性对疾病诊断和治疗监测的意义。方法应用CLQ-1图象分析系统及其配套细胞培养、银染等试剂和方法,分析了84例恶性肿瘤、炎症及免疫等疾病患者和16例临床健康人的外周血淋巴细胞rDNA转录活性(以核仁组成区嗜银蛋白银染强度来表达)。结果恶性肿瘤和白血病患者rDNA转录活性明显降低,炎症患者明显增强,与健康人比较,差异有显著意义;免疫疾病和其他非恶性疾病患者与健康人相近,差异无统计学意义。结论rDNA转录活性分析可能成为肿瘤诊断及其与炎症及免疫等疾病鉴别诊断和治疗监测的新指标。
ObjectiveForlearningtheclinicalsignificanceofrDNAtranscriptionactivityofpe-ripheralbloodlymphocyte(PBL).MethodsrDNAtranscriptionactivitywasdetectedbyacelim-ageanalysisofAg-NORin16casesofnormalvolunteerand84patients(cancer50,immunodisease6,inflammation9andothernon-perniciousdisease19).ResultsrDNAtranscriptionactivityofPBLwasdecreasedinpatientswithcancerandleukemiaandincreasedinpatientswithinflammation,showingastatisticalysignificantdifference.Theresultsinpatientswithimmunodiseasesandothernon-perniciousdiseasesshowednosignificantdiference,ascomparedwithnormalcontrols.Conclu-sionsTheanalysisofrDNAtranscriptionactivityofPBLmayserveasanewtumourmarkerfordi-agnosisanddifferentialdiagnosisclinicaly.