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Hot water-extracted Lycium barbarum and Rehmannia glutinosa inhibit proliferation and induce apoptosis of hepatocellular carcinoma cells 被引量:31

Hot water-extracted Lycium barbarum and Rehmannia glutinosa inhibit proliferation and induce apoptosis of hepatocellular carcinoma cells
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摘要 AIM: To investigate the effect of hot water-extracted Lydurn barbarum (LBE) and Rehrnannia glutinosa (RGE) on cell proliferation and apoptosis in rat and/or human hepatocellular carcinoma (HCC) cells. METHODS: Rat (H-4-Ⅱ-E) and human HCC (HA22T/ VGH) cell lines were incubated with various concentrations (0-10 g/L) of hot water-extracted LBE and RGE. After 6-24 h incubation, cell proliferation (n = 6) was measured by a colorimetric method. The apoptotic cells (n = 6) were detected by flow cytometry. The expression of p53 protein (n = 3) was determined by SDS-PAGE and Western blotting. RESULTS: Crude LBE (2-5 g/L) and RGE (2-10 g/L) dose-dependently inhibited proliferation of H-4-Ⅱ-E cells by 11% (P 〈 0.05) to 85% (P 〈 0.01) after 6-24 h treatment. Crude LBE at a dose of 5 g/L suppressed cell proliferation of H-4-Ⅱ-E cells more effectively than crude RGE after 6-24 h incubation (P 〈 0.01). Crude LBE (2-10 g/L) and RGE (2-5 g/L) also dose-dependently inhibited proliferation of HA22T/VGH cells by 14%-43% (P 〈 0.01) after 24 h. Crude LBE at a dose of 10 g/L inhibited the proliferation of HA22T/VGH cells more effectively than crude RGE (56.8% + 1.6% vs 70.3% + 3.1% of control, P = 0.0003 〈 0.01). The apoptotic cells significantly increased in H-4-Ⅱ-E cells after 24 h treatment with higher doses of crude LBE (2-5 g/L) and RGE (5-10 g/L) (P 〈 0.01). The expression of p53 protein in H-4-Ⅱ-E cells was 119% and 143% of the control group compared with the LBE-treated (2, 5 g/L) groups, and 110% and 132% of the control group compared with the RGE -treated (5, 10 g/L) groups after 24 h. CONCLUSION: Hot water-extracted crude LBE (2-5 g/L) and RGE (5-10 g/L) inhibit proliferation and stimulate p53-mediated apoptosis in HCC cells. 瞄准:在老鼠或人的肝细胞癌(HCC ) 在房间增长和 apoptosis 上调查热提取水的 Lycium 倒刺海芋属植物(LBE ) 和 Rehmannia glutinosa (RGE ) 的效果房间。方法:老鼠(H-4-II-E ) 和 HCC (HA22T/VGH ) 房间衬里的人与热提取水的 LBE 和 RGE 的各种各样的集中(0-10 g/L ) 被孵化。在 6-24 h 孵化以后,房间增长(n = 6 ) 被比色法测量。apoptotic 房间(n = 6 ) 被流动血细胞计数检测。p53 蛋白质的表示(n = 3 ) 被 SDS 页并且西方的弄污决定。结果:粗略的 LBE (2-5 g/L ) 和 RGE (2-10 g/L ) dose-dependently 在 11% 禁止了 H-4-II-E 房间的增长(P < 0.05 ) 到 85%(P < 0.01 ) 在 6-24 h 处理以后。在 5 g/L 的剂量的粗略的 LBE 在 6-24 h 孵化以后比粗略的 RGE 更有效地压制了 H-4-II-E 房间的房间增长(P < 0.01 ) 。粗略的 LBE (2-10 g/L ) 和 RGE (2-5 g/L ) dose-dependently 也由 14%-43% 禁止了 HA22T/VGH 房间的增长(P < 0.01 ) 在 24 h 以后。在 10 g/L 的剂量的粗略的 LBE 比粗略的 RGE 更有效地禁止了 HA22T/VGH 房间的增长(56.8%+/-1.6% 对 70.3%+/-3.1% 控制, P = 0.0003 < 0.01 ) 。apoptotic 房间显著地与粗略的 LBE (2-5 g/L ) 和 RGE (5-10 g/L ) 的更高的剂量在 24 h 处理以后在 H-4-II-E 房间增加了(P < 0.01 ) 。在 H-4-II-E 房间的 p53 蛋白质的表达式是 119% 控制组和 143% 与相比对待 LBE (2, 5 g/L ) 组,和 110% 控制和 132% 组与 RGE 相比对待(5, 10 g/L ) 在 24 h 以后的组。结论:热提取水的粗略的 LBE (2-5 g/L ) 和 RGE (5-10 g/L ) 禁止增长并且在 HCC 房间刺激调停 p53 的 apoptosis。
出处 《World Journal of Gastroenterology》 SCIE CAS CSCD 2006年第28期4478-4484,共7页 世界胃肠病学杂志(英文版)
基金 Supported by the National Science Council, No. NSC92-2320-B038-032 Taipei Medical University-Wan Fang Hospital, No. 93TMU-WFH-19
关键词 Lycium barbarum extract Rehmannia glutinosa extract PROLIFERATION APOPTOSIS Hepatocellular carcinoma 地黄 细胞凋亡 肝细胞癌 肿瘤细胞
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