大鼠睾丸支持细胞的原代培养、鉴定与抑制素的表达

Primary Culture and Identification of Rat Testicular Sertoli Cells in Vitro and Expression of Inhibin Inside the Cells

目的：研究大鼠睾丸支持细胞体外培养的有效方法，探讨支持细胞抑制素的表达。方法：对18—22d的大鼠睾丸支持细胞进行分离培养，观察支持细胞体外培养的生物学特性，用免疫组化方法检测支持细胞抑制素α、βA、ββ亚基的表达。结果：在体外成功培养出大鼠睾丸支持细胞，细胞纯度可达到90％以上。支持细胞中抑制素（INH）α、βA、Bβ亚基均为强阳性表达。结论：建立了大鼠睾丸支持细胞纯化培养方法，支持细胞中INHα、βA、ββ亚基表达均为强阳性表达，证实支持细胞能够合成、分泌INH。

Objective： To establish a primary culture method of rat testicular Sertoli cells and study the expressions of inhibin in Sertoli cells. Methods：Sertoli cells aged 18-22 days were separated and cultured from rat testes and the biological characteristics of Sertoli cells were observed,and the expressions of inhibin α, βA and βB subunits in Sertoli cells were detected by means of immunohistochemistry. Results：Sertoli cells in rat testicles were successfully cultured in vitro in the present study and the degree of purity in rat Sertoli cells was more than 90% of the total cells. Sertoli cells in vitro were positive for Inhibin α, βA and ~B subunits. Conclusion： In vitro culture method of rat Sertoli cells was successfully established. Sertoli cells in vitro were positive for inhibin α, βA and βB subunits and rat testicular Sertoli cells were capable of producing inhibin and proven to be the major source of inhibin.