中华大蟾蜍蟾酥与蟾皮化学成分的比较分析

A comparison study on the chemical constituents of Venenum Bufonis and the skin of toad

目的:比较蟾酥与蟾皮的化学成分。方法:采用薄层色谱法(TLC)和高效液相色谱法(HPLC)。结果:蟾酥及蟾皮中的二烯内酯成分大多数相同,但蟾酥中的种类少而含量更集中;吲哚生物碱类有4个主要成分相同,另一吲哚生物碱脱氢蟾蜍色胺,在蟾皮中的相对含量较高,而蟾酥中很低;且初步判断蟾酥中甾醇类主要为β-谷甾醇,而蟾皮中主要为胆甾醇和胆甾醇棕榈酸酯。结论:蟾酥与蟾皮化学成分存在差异。

Object： To compare the chemical constituents of Venenum Bufonis （VB） with the skin of toad＇s （ST）. Methods： TLC and HPLC methods were used. Results： Most of the bufadienolides were the same in VB and ST, but those in VB were more concentrative; Four main indole - alkaloids were the same, the content of another dehydrobufotenine was higher in ST, but very low in VB; β - sitosterol was identified in VB, but Cholesterol and cholesteryl palmitate in ST. Conclusion： The chemical constituents between VB and ST had some difference.